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<strong><em><font size="3"><em><strong><font size="3">No7. Preparation of stock solution</font><br />
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<td bgcolor="#cccccc"><font size="2"><strong> 1.5M Tris (pH 8.8</strong><em>)</em></font></td> <td bgcolor="#cccccc"><font size="2"><em> </em>Dissolve 181.5g of Tris base in 800ml of distilled H2O.<br />
Adjust the pH to 8.8 with concentrated HCl.<br />
Adjust the volume to 1 liter.<br />
Dispense in convenient volumes and sterilize by autoclaving.<br />
Store at room temperature.</font></td>
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<td><font size="2"><em> </em><strong>1.0M Tris (pH 6.8</strong><em>)</em></font></td> <td><font size="2"><em> </em>Dissolve 12.1g of Tris base in 80ml of distilled H2O.<br />
Adjust the pH to 6.8 with concentrated HCl.<br />
Adjust the volume to 100 ml.<br />
Dispense in convenient volumes and sterilize by autoclaving.<br />
Store at room temperature.</font></td>
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<td bgcolor="#cccccc"><font size="2"><em> </em><strong>10% Sodium dodecyl sulfate (SDS)</strong></font></td>
<td bgcolor="#cccccc"><font size="2"> Dissolve 10g of SDS in 100ml of distilled H2O.<br />
Store at room temperature. </font></td>
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No8. Solutions for Tris/ glycine SDS-polyacrylamide gel electrophoresis<br />
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