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구체적인 단백질 실험 프로토콜

3,024 bytes added, 02:52, 19 February 2007
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<tdbgcolor="#0066ff"><strong>&nbsp;<font color="#ffff99">6%</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;5ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;10ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;15ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;20ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;25ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;30ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;40ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;50ml</font></strong></td>
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<tdbgcolor="#cccccc">&nbsp;<strong>H2O<br />
&nbsp;30% Acrylamide mix<br />
&nbsp;1.5M Tris (pH 8.8)<br />
&nbsp;10% APS<br />
&nbsp;TEMED</strong></td>
<tdbgcolor="#cccccc">&nbsp;2.6<br />
&nbsp;1.0<br />
&nbsp;1.3<br />
&nbsp;0.05<br />
&nbsp;0.004</td>
<tdbgcolor="#cccccc">&nbsp;5.3<br />
&nbsp;2.0<br />
&nbsp;2.5<br />
&nbsp;0.1<br />
&nbsp;0.008</td>
<tdbgcolor="#cccccc">&nbsp;7.9<br />
&nbsp;3.0<br />
&nbsp;3.8<br />
&nbsp;0.15<br />
&nbsp;0.012</td>
<tdbgcolor="#cccccc">&nbsp;10.6<br />
&nbsp;4.0<br />
&nbsp;5.0<br />
&nbsp;0.2<br />
&nbsp;0.016</td>
<tdbgcolor="#cccccc">&nbsp;13.2<br />
&nbsp;5.0<br />
&nbsp;6.3<br />
&nbsp;0.25<br />
&nbsp;0.02</td>
<tdbgcolor="#cccccc">&nbsp;15.9<br />
&nbsp;6.0<br />
&nbsp;7.5<br />
&nbsp;0.3<br />
&nbsp;0.024</td>
<tdbgcolor="#cccccc">&nbsp;21.2<br />
&nbsp;8.0<br />
&nbsp;10.0<br />
&nbsp;0.4<br />
&nbsp;0.032</td>
<tdbgcolor="#cccccc">&nbsp;26.5<br />
&nbsp;10.0<br />
&nbsp;12.5<br />
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<tdbgcolor="#0066ff">&nbsp;<strong><font color="#ffff99">8%</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;5ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;10ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;15ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;20ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;25ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;30ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;40ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;50ml</font></strong></td>
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<tdbgcolor="#0066ff">&nbsp;<strong><font color="#ffff99">10%</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;5ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;10ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;15ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;20ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;25ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;30ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;40ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;50ml</font></strong></td>
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<tdbgcolor="#cccccc">&nbsp;<strong>H2O<br />
&nbsp;30% Acrylamide mix<br />
&nbsp;1.5M Tris (pH 8.8)<br />
&nbsp;10% APS<br />
&nbsp;TEMED</strong></td>
<tdbgcolor="#cccccc">&nbsp;2.0<br />
&nbsp;1.7<br />
&nbsp;1.3<br />
&nbsp;0.05<br />
&nbsp;0.002</td>
<tdbgcolor="#cccccc">&nbsp;4.0<br />
&nbsp;3.3<br />
&nbsp;2.5<br />
&nbsp;0.1<br />
&nbsp;0.006</td>
<tdbgcolor="#cccccc">&nbsp;5.9<br />
&nbsp;5<br />
&nbsp;3.8<br />
&nbsp;0.15<br />
&nbsp;0.006</td>
<tdbgcolor="#cccccc">&nbsp;7.9<br />
&nbsp;6.7<br />
&nbsp;5.0<br />
&nbsp;0.2<br />
&nbsp;0.008</td>
<tdbgcolor="#cccccc">&nbsp;9.9<br />
&nbsp;8.3<br />
&nbsp;6.3<br />
&nbsp;0.25<br />
&nbsp;0.01</td>
<tdbgcolor="#cccccc">&nbsp;11.9<br />
&nbsp;10.0<br />
&nbsp;7.5<br />
&nbsp;0.3<br />
&nbsp;0.012</td>
<tdbgcolor="#cccccc">&nbsp;15.8<br />
&nbsp;13.3<br />
&nbsp;10.0<br />
&nbsp;0.4<br />
&nbsp;0.016</td>
<tdbgcolor="#cccccc">&nbsp;19.8<br />
&nbsp;16.7<br />
&nbsp;12.5<br />
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<tdbgcolor="#0066ff">&nbsp;<strong><font color="#ffff99">12%</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;5ml </font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;10ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;15ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;20ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;25ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;30ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;40ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;50ml</font></strong></td>
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<tdbgcolor="#0066ff">&nbsp;<strong><font color="#ffff99">15%</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;5ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;10ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;15ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;20ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;25ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;30ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;40ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;50ml</font></strong></td>
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<tdbgcolor="#cccccc">&nbsp;<strong>H2O<br />
&nbsp;30% Acrylamide mix<br />
&nbsp;1.5M Tris (pH 8.8)<br />
&nbsp;10% APS<br />
&nbsp;TEMED</strong></td>
<tdbgcolor="#cccccc">&nbsp;1.1<br />
&nbsp;2.5<br />
&nbsp;1.3<br />
&nbsp;0.05<br />
&nbsp;0.002</td>
<tdbgcolor="#cccccc">&nbsp;2.3<br />
&nbsp;5.0<br />
&nbsp;2.5<br />
&nbsp;0.1<br />
&nbsp;0.004</td>
<tdbgcolor="#cccccc">&nbsp;3.4<br />
&nbsp;7.5<br />
&nbsp;3.8<br />
&nbsp;0.15<br />
&nbsp;0.006</td>
<tdbgcolor="#cccccc">&nbsp;4.6<br />
&nbsp;10.0<br />
&nbsp;5.0&nbsp;<br />
&nbsp;0.2<br />
&nbsp;0.008</td>
<tdbgcolor="#cccccc">&nbsp;5.7<br />
&nbsp;12.5<br />
&nbsp;6.3<br />
&nbsp;0.25<br />
&nbsp;0.01</td>
<tdbgcolor="#cccccc">&nbsp;6.9<br />
&nbsp;15.0<br />
&nbsp;7.5<br />
&nbsp;0.3<br />
&nbsp;0.012</td>
<tdbgcolor="#cccccc">&nbsp;9.2<br />
&nbsp;20.0<br />
&nbsp;10.0<br />
&nbsp;0.4<br />
&nbsp;0.016</td>
<tdbgcolor="#cccccc">&nbsp;11.4<br />
&nbsp;25.0<br />
&nbsp;12.5<br />
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<tdbgcolor="#0066ff">&nbsp;<strong><font color="#ffff99">STACK</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;1ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;2ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;3ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;4ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;5ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;6ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;8ml</font></strong></td> <tdbgcolor="#0066ff"><strong><font color="#ffff99">&nbsp;10ml</font></strong></td>
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