염석법

From Opengenome.net

염석은 용해도가 큰 염을 단백질 혼합액에 가하여 
이온강도의 변화로 인해 단백질을 침전시키는 것이다.

 

                                                                                                                  황산암모늄 첨가 도표


 

 

Final concentration of ammonium sulfate,

% saturation

10

20

25

30

33

35

40

45

50

55

60

65

70

75

80

90

100

Grams solid ammonium sulfate to be added to 11. of solution

 Initial concentration of

ammonium sulfate,% saturation

0

56

114

144

176

196

209

243

277

313

351

390

430

472

516

561

662

767

10

 

57

86

118

137

150

183

216

251

288

326

365

406

449

494

592

694

20

 

29

39

78

91

123

155

189

225

262

300

340

382

424

520

619

25

 

30

49

61

93

125

158

193

230

267

307

348

390

485

583

30

 

19

30

62

94

127

162

198

235

273

314

356

449

546

33

 

12

43

74

107

142

177

214

252

292

333

426

522

35

 

31

63

94

129

164

200

238

278

319

411

506

40

 

31

63

97

132

168

205

245

285

375

469

45

 

62

65

99

134

171

210

250

339

431

50

 

33

66

101

137

176

214

302

392

55

 

33

67

103

141

179

264

353

60

 

34

69

105

143

227

314

65

 

34

70

107

190

275

70

 

35

72

153

237

75

 

36

115

198

80

 

77

157

90

 

79

                                                                     -용액 1ℓ에 가할 고체상 황산암모늄의 g수