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제한효소
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<p> [[핵산 분해효소]]의 한 종류이다. <br /> 유전공학에서 재조합 DNA(디옥시리보핵산)를 만들기 위해서 사용하는 특수한 효소이며 제한이란, 이질(<span onmouseover="tooltip_on('','이 : 다를 이, ㉠다르다 ㉡괴이하다 ㉢이상하게 여기다 ㉣재앙')" onmouseout="tooltip_off()">異</span><span onmouseover="tooltip_on('','질 : 바탕 질, 폐백 지, ㉠바탕 ㉡근본 ㉢볼모 ㉣묻다 ㉤바르게 하다 ㉥전당잡다 ㉦이루다 ㉧질박하다 ⓐ폐백(贄) (지)')" onmouseout="tooltip_off()">質</span>)의 숙주(<span onmouseover="tooltip_on('','숙 : 잠잘 숙, 성수 수, ㉠자다 ㉡묵다 ㉢지키다 ㉣본디 ㉤숙직 ㉥당직 ⓐ성수 (수)')" onmouseout="tooltip_off()">宿</span><span onmouseover="tooltip_on('','주 : 주인 주, ㉠주인 ㉡임금 ㉢우두머리 ㉣주체 ㉤자신 ㉥위패 ㉦주되다')" onmouseout="tooltip_off()">主</span>) 중에서 증식한 DNA의 침입을 받았을 때 그 DNA와 자기의 DNA를 식별하고 분해해 버리는 현상을 말한다, 그리고 그런 작용을 하는 효소를 제한효소라고 한다. 이와 반대로 제한효소가 인식하는 염기배열 중의 특정한 염기를 메틸화하여 제한을 받지 않는 상태로 DNA를 일시적으로 변화시키는 현상을 수식(<span onmouseover="tooltip_on('','수 : 닦을 수, ㉠닦다 ㉡꾸미다 ㉢고치다')" onmouseout="tooltip_off()">修</span><span onmouseover="tooltip_on('','식 : 꾸밀 식, ㉠꾸미다 ㉡꾸밈 ㉢가선(의복 가장자리를 딴 감으로 가늘게 두른 선)')" onmouseout="tooltip_off()">飾</span>)이라고 하고, 이런 작용을 하는 효소를 수식효소라고 한다.<br /><br /> 제한효소는 그 특성(절단양식이나 활성발현인자 등)에 의해 Ⅰ, Ⅱ, Ⅲ형의 3군(<span onmouseover="tooltip_on('','군 : 무리 군, ㉠무리 ㉡떼 ㉢벗 ㉣많은 ㉤여럿의 ㉥많다 ㉦떼를 짓다 ㉧모이다 ㉨모으다')" onmouseout="tooltip_off()">群</span>)으로 대별된다. Ⅰ형 효소는 활성발현에 APT, <em>S</em>-아데노실메티오닌 및 마그네슘이온(Mg<sup>2+</sup>)을 필요로 하고, DNA 속의 인식염기배열로부터 떨어진 부위를 절단하는데 그 위치는 일정하지 않아 유전공학 적으로 사용이 잘 되지 않는다. Ⅱ형효소는 유전공학에서 널리 사용되는 것으로 활성발현에 마그네슘이온을 필요로 하여 DNA분자의 특정한 염기배열을 인식하고 그 부위 또는 인식염기배열로부터 일정 염기 떨어진 인접부위를 정확하게 절단하는게 가장 큰 특징이다. 그리고 이 효소의 인식염기배열은 3~6개의 2회전 대칭구조를 취하고 있는 경우가 많다. Ⅲ형효소는 활성발현에 APT와 마그네슘이온을 필요로 하고, DNA 속의 인식염기배열로부터 수십 염기 떨어진 부위를 절단하기 때문에 잘 사용이 안된다. <br /><br /></p>
<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> </span><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'"><font face="굴림">※</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">제한효소</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> (restriction enzyme) <br /></span></font><font size="3"><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">를</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">자른다는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">것은</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> nucleotide</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">들이</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">연결되어</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">있는</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> phosphodiester </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">결합을</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">끊어내는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">것</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">으로</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">이런</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">역할을</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">하는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">효소를</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> nuclease</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">라고</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">하며</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">바깥쪽을</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">자르는</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> exonuclease</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">와</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">안쪽을</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">자르는</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> endonuclease</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">가</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">있다</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">제한효소</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> (restriction enzyme, restriction endonuclease)</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">는</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> endonuclease</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">로</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">에</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">존재하는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">특정한</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">염기서열을</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">인식하고</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">인식한</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">서열부위</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">또는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">그</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">근처에</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">존재하는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">특정부위를</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">절단한다</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">. <br /></span></font><font size="3"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">제한효소의</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> 3</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">가지</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> type</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">중</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">실험실에서</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">사용되는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">것은</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">대부분</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> Type II restriction enzyme</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">으</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">로</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">인식부위와</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">절단부위가</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">모두</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">특이성이</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">있는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">제한효소로</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> 4</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">개</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">, 5</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">개</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">, </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">또는</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> 6</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">개의</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">염기서열</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">을</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">인식한다</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">제한효소의</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">명명은</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">발견된</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">세균의</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">이름</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">및</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> serotype</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">에</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">따라서</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">붙이게</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">된다</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">. </span></font><font size="3"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">제한효소가</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">인식할</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">수</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">있는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">부위는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">앞으로</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">읽으나</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">뒤로</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">읽으나</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">염기서열이</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">같은</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">모양</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> (palindrome)</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">을</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">하고</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">있는</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">것이</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">특징이다</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">잘린</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">후의</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">모양은</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> 5'-overhang cohesive end, 3'-overhang cohesive end (sticky end) </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">또는</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> blunt end</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">가</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">만들어질</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">수</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">있으므로</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">목적</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">에</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">따라서</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">잘</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">선택해서</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">사용해야</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">한다</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">.<br /> <br /></span></font><font size="3"><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">예</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">1) EcoR I </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">효소의</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">절단</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">부위</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">: 5'-overhang cohesive end </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">형성</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> <br /></span></font><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""><font size="3"> 5'--GAATTC--3' → 5'--G AATTC--3' </font></span></p><p> </p><p><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""><font size="3"> 3'--CTTAAG--5' 3'--CTTAA G--5' <br /><pbr /> <font size="3"><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">예</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">2) Sac I </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">효소의</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">절단</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">부위</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">: 3'-overhang cohesive end </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">형성</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> <br /span></fontspan></pfont></font></span></p><p> </p><p><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""><font size="3"> 5'--GAGCTC--3' → 5'--GAGCT C--3' </font></span></p><p> </p>
<p><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""><font size="3"> 3'--CTCGAG--5' 3'--C TCGAG--5' </font></span></p>
<p> </p><p><font size="3"><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">예</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">3) Sma I </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">효소의</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">절단</font></span><span style="FONT-FAMILY: "Times New Roman""> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">부위</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman"">: blunt end </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'"><font face="굴림">형성</font></span><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""> </span></font></p><p> </p>
<p><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""><font size="3"> 5'--CCCGGG--3' → 5'--CCC GGG--3' </font></span></p>
<p> </p><p><span lang="EN-US" style="FONT-FAMILY: "Times New Roman""><font size="3"> 3'--GGGCCC--5' 3'--GGG CCC--5' </font></span></p><p> <br /p><p><br /></p>
