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64 bytes added, 21:43, 25 November 2011
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<p>&nbsp;</p>
<h2><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">Method of Sample (gDNA) Preparation for Sending out</span>&nbsp;</h2>
<p>&nbsp;</p>
<p><span style="font-size: small"><b><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1.&nbsp;Sample(gDNA) Preparation</span></b></span></p>
<p>1)&nbsp;&nbsp;DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p>
<p>2)&nbsp;&nbsp;Do Not Dissolve in Water</p>
<p>&nbsp;</p>
<p><b><span style="font-size: small"><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2.&nbsp;Sample(gDNA) Requirement</span></span></b></p>
<p>1)&nbsp;&nbsp;&nbsp;Amount and concentration spec.&nbsp;: over 20 ug of gDNA with &gt;50 ng/ul</p>
<p>b.&nbsp;&nbsp;Whole exome sequencing (50X)</p>
<p>c.&nbsp;&nbsp;Genotyping</p>
<p>2)&nbsp;&nbsp;Sample(gDNA) should be&nbsp;: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p><p>&nbsp;</p>
<p><b><span style="font-size: small"><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3.&nbsp;Sample(gDNA) Packing Information&nbsp;</span></span></b></p>
<p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
<p>5)&nbsp;&nbsp;Put into the 50 ml Falcon tube and close the cap</p>
<p>6)&nbsp;&nbsp;Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
<p>&nbsp;</p>
<p><b><span style="font-size: small"><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4.&nbsp;Requirement of Sample(gDNA) Information</span></span></b></p>
<p>1)&nbsp; Sample type: DNA</p>
<p>9)&nbsp; OD260/280</p>
<p>10)&nbsp; OD260/230</p>
<p>&nbsp;</p>
<p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p>
<p>1.<span style="color: #800000"><a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p>
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