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<h2><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">Method of Sample (gDNA) Preparation for Sending out</span> </h2>
<p> </p>
<p><span style="font-size: small"><b><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1. Sample(gDNA) Preparation</span></b></span></p>
<p>1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p>
<p>2) Do Not Dissolve in Water</p>
<p> </p>
<p><b><span style="font-size: small"><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2. Sample(gDNA) Requirement</span></span></b></p>
<p>1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul</p>
<p>b. Whole exome sequencing (50X)</p>
<p>c. Genotyping</p>
<p>2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p><p> </p>
<p><b><span style="font-size: small"><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3. Sample(gDNA) Packing Information </span></span></b></p>
<p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
<p>5) Put into the 50 ml Falcon tube and close the cap</p>
<p>6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
<p> </p>
<p><b><span style="font-size: small"><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4. Requirement of Sample(gDNA) Information</span></span></b></p>
<p>1) Sample type: DNA</p>
<p>9) OD260/280</p>
<p>10) OD260/230</p>
<p> </p>
<p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p>
<p>1.<span style="color: #800000"><a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p>