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Korean Personal Genome Project
Contents
Introduction
Mission
- to function as Korean center for PGP (Harvard Medical School, George Church lab)
- to build a standard Korean genetic information database
- to activate the participative genome research
- to develop genome sequencing and analysis technologies in the age of personal genetic information services
- to establish an open community if sharing ethical, social, and legal issued in regard to genetic information
Research
- Korean reference genome project
- Rare diseases reference genome project
- HLA alleles reference standard data production project
Participation
Genetic Information Participation
It is a public participation. The human genetics education, screening and participant`s willingness determine an individual's participation.
Research Participation
People who have research experiences in the field of human genetics, or who are willing to contribute to KPGP can participate through screening.
Company Participation
Institutes and companies could participate. Sponsorship can greatly help KPGP`s activation.
Human genome researches have developed rapidly since the human genome project was completed in 2003. Recently large-scale production of human genome data is available with the advent of Next Generation Sequencing (NGS).
Genome Research Foundation (GRF), as a non-profit foundation, determines to open Korean genome data and the current available data of human genome sequence and analysis for free to allow researchers to study. Under the circumstances, OPEN KPGP started on 2011.
Genetic Data Usage Agreement for Research
1) Data Type
Every derived genetic information should be approved by relevant facility board.
1. Genetic information data- Individual's sequenced DNA and analyzed data.
2. Clinical information data- Clinical information does not include family tree, Phenotype and family medical history.
2) The Commission Process
1. Bioethics committee of Genome Research Foundation will make a decision through policy reviews and case consultation.
2. The Standards Commission
This commission should be controlled by Korea National Institute for Bioethics Policy.
Research, associate with potential social risks, eugenical problem and discrimination on the basis of genetic information when it comes to any aspects of physical looking, should be forbidden.
3. The Commission Process
Research project and IRB document, approved in each countries, will be required. If there is no provision for IRB approval, User must agree with additional consent documents that embodies the purpose of the data.
4. Evaluation (It will take at least one week)
3) Policy Agrement
Informed consent shall be documented by the use of a written consent form approved by the IRB, and signed by the subject or the subject's legally authourized representative.
And if necessary, the committee may request, require or otherwise obtain detailed investigation.
Data Release
Any additional costs to the subject that may result from participation in the research.
4) Data Source Agrement
The Genome Research Foundation should review and approve specifying the conditions under which data may be accepted, and ensuring adequate provisions to protect the privacy of subjects and maintain the confidentiality of data.
To cite the data source in any publications or research based upon these data, and to provide a copy of any publications, the following citation should be included in any research reports, papers, or publications based on these data:
Produced and distributed data should have references in Acknowledgement, Methods, Abstract.
How to download
After accessing to the OPEN KPGP website, select the desired data from DATA DOWNLOAD to download it through the Web and FTP.
Please understand that the downloading may be very slow due to Network load. (The speed of downloading 20 people’s data through KT cloud is stable, please use it as an alternative.)
Hard disk request
You can get data hard disks by requesting it to the administrator. However, the hard disk and processing costs will occur.
Cost:
- Hard disks
- Operating costs (labor and server equipment operating costs).
- Shipping : door to door delivery(domestic only) / international shipping : please contact. (info@kpgp.kr)
(The data charges are variable based on the quantity and price of the hard disk, one hard disk can contain different numbers of samples.)
1) Raw sequencing data
| Sample no. | Cost(excluding V.A.T) |
| ~10 | 115,000 (KRW) |
| ~20 | 170,000 (KRW) |
| ~30 | 260,000 (KRW) |
| ~40 | 340,000 (KRW) |
2) Data included : raw sequencing data, aligned data, genome variation, nonsynonymous SNVs
| Sample no. | Cost(excluding V.A.T) |
| ~10 | 310,000 (KRW) |
| ~20 | 530,000 (KRW) |
| ~30 | 750,000 (KRW) |
| ~40 | 880,000 (KRW) |
Date for pricing: 2011.09
Method of Sample (gDNA) Preparation for Sending out
1. Sample(gDNA) Preparation
1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.
2) Do Not Dissolve in Water
2. Sample(gDNA) Requirement
1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul
This amount is required for
a. Whole genome sequencing (30X)
b. Whole exome sequencing (50X)
c. Genotyping
2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination
3. Sample(gDNA) Packing Information
This way is only available when DNA is dissolved in TE or Tris buffer.
1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service
2) Mark sample information on tube (concentration, sample name…what you want)
3) Seal the cap of tube with parafilm well
4) Wrap the tube with paper towel or equivalent
5) Put into the 50 ml Falcon tube and close the cap
6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent
4. Requirement of Sample(gDNA) Information
1) Sample type: DNA
2) Condition: DNA in TE buffer or EB buffer or equivalent. Not in water
3) Gel image of DNA electrophoresis
4) Species
5) No. of Tubes
6) Concentration (ng/μl)
7) Volume (μl)
8) Total Quantity (μg)
9) OD260/280
10) OD260/230
Please download and refer to the following documents for information on gDNA methods of preparation and delivery.
1.Method of Sample (gDNA) Preparation for Sending out
How it works
Data Usage
1. The genome data is provided by Genome Research Foundation. Anyone can use the data freely.
2. People who would like to acquire further information about the data for research publication and collaboration, please contact us.
Contact: info@pgi.re.kr / 82-43-235-8687
