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가수분해(加水分解)는 화학 반응시, 결합 부분에 물이 끼어들어가면서 원래 하나였던 큰 분자가 두개로 분해되는 반응을 말한다. 생명체 내에서는 대부분의 결합들이 가수분해로 깨질 수 있다. 대표적인 생명체 내 가수분해 반응들은 다음과 같다.

• 아데노신 삼인산 분해 : 생명체가 사용하는 에너지를 제공한다.
• 탄수화물, 단백질의 소화 : 각각 글리코시드결합과 펩타이드 결합을 깨 이당류/단당류 및 아미노산으로 쪼개 장에서의 흡수를 돕는다.