0
edits
Changes
no edit summary
<p> <br />
<br />
<strong><font size="3"><em>No9. David's life chart2<br />
</em></font></strong><br />
<table style="WIDTH: 788px; HEIGHT: 363px" cellspacing="1" cellpadding="1" width="788" summary="" border="1">
</table>
</p>
<br />
<br />
<strong><em><font size="3">David's life chart 3<br />
</font></em></strong><hr />
<strong><font size="3">Some useful nucleotide dimensions</font></strong><hr />
<font size="2"> </font><strong><font size="2">1cm of DNA ≈3 * 10의 6제곱 nucleotides<br />
</font></strong>
<table style="WIDTH: 789px; HEIGHT: 123px" cellspacing="1" cellpadding="1" width="789" summary="" border="1">
<tbody>
<tr>
<td bgcolor="#0066ff"><strong><font size="2"> <font color="#ffff99">Organism</font></font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2"> Base pairs/<br />
haploid genome</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2"> Base pairs/<br />
diploid genome</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2"> Length / cell</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2"> Mass</font></strong></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2"> Human</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 3 * 10의 9제곱</font></td>
<td bgcolor="#cccccc"><font size="2"> 6 * 10의 9제곱</font></td>
<td bgcolor="#cccccc"><font size="2"> 2 meters (diploid)</font></td>
<td bgcolor="#cccccc"><font size="2"> 6 pg</font></td>
</tr>
<tr>
<td><strong><font size="2"> Fly</font></strong></td>
<td><font size="2"> 1.65 * 10의 8제곱</font></td>
<td><font size="2"> 3.3 * 10의 8제곱</font></td>
<td><font size="2"> 100 cm (diploid)</font></td>
<td><font size="2"> 0.3 pg</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2"> Yeast</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 1.35 * 10의 7제곱</font></td>
<td bgcolor="#cccccc"><font size="2"> 2.7 * 10의 7제곱</font></td>
<td bgcolor="#cccccc"><font size="2"> 10 cm (diploid)</font></td>
<td bgcolor="#cccccc"><font size="2"> 0.03 pg</font></td>
</tr>
<tr>
<td><strong><em><font size="2"> E.coli</font></em></strong></td>
<td><font size="2"> 4.7 * 10의 6제곱</font></td>
<td><font size="2"> -</font></td>
<td><font size="2"> 1.5 cm (haploid)</font></td>
<td><font size="2"> 0.0045 pg</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2"> SV40</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 5 * 10의 3제곱</font></td>
<td bgcolor="#cccccc"><font size="2"> - </font></td>
<td bgcolor="#cccccc"><font size="2"> 1.7 nm</font></td>
<td bgcolor="#cccccc"><font size="2"> 0.000006 pg</font></td>
</tr>
</tbody>
</table>
<br />
<br />
<br />
<strong><font size="3">Some useful cell dimensions<br />
<br />
</font></strong>
<table style="WIDTH: 787px; HEIGHT: 223px" cellspacing="1" cellpadding="1" width="787" summary="" border="1">
<tbody>
<tr>
<td bgcolor="#0066ff"><strong> <font color="#ffff99" size="2">Organism</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2"> Dimensions</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2"> Volume</font></strong></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><em><font size="2"> S.cerevisiae</font></em></strong></td>
<td bgcolor="#cccccc"><font size="2"> 5 um</font></td>
<td bgcolor="#cccccc"><font size="2"> 66 um³</font></td>
</tr>
<tr>
<td><strong><em><font size="2"> S. pombe</font></em></strong></td>
<td><font size="2"> 2 * 7 um</font></td>
<td><font size="2"> 22 um³</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2"> Mammalian cell</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 10-20 um</font></td>
<td bgcolor="#cccccc"><font size="2"> 500-4,000 um³</font></td>
</tr>
<tr>
<td><strong><em><font size="2"> E.coli</font></em></strong></td>
<td><font size="2"> 1 * 3 um</font></td>
<td><font size="2"> 2 um³</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2"> Mammalian mitochondrion</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 1 um </font></td>
<td bgcolor="#cccccc"><font size="2"> 0.5 um³</font></td>
</tr>
<tr>
<td><strong><font size="2"> Mammalian nucleus</font></strong></td>
<td><font size="2"> 5-10 um</font></td>
<td><font size="2"> 66-500 um³</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2"> Plant chloroplast</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 1 * 4 um</font></td>
<td bgcolor="#cccccc"><font size="2"> 3 um³ </font></td>
</tr>
<tr>
<td><strong><font size="2"> Bacteriophage lambda</font></strong></td>
<td><font size="2"> 50 nm (head only)</font></td>
<td><font size="2"> 6.6 * 10의 -5제곱 um³</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2"> Ribosome</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 30 nm diameter</font></td>
<td bgcolor="#cccccc"><font size="2"> 1.4 * 10의 -5제곱 um³</font></td>
</tr>
<tr>
<td><strong><font size="2"> Globular monomeric protein</font></strong></td>
<td><font size="2"> 5 nm diameter</font></td>
<td><font size="2"> 6.6 * 10의 -8제곱 um³</font></td>
</tr>
</tbody>
</table>
<br />
<br />
<br />
<strong><font size="3">Some useful concentrarions</font></strong><br />
<br />
<table style="WIDTH: 787px; HEIGHT: 83px" cellspacing="1" cellpadding="1" width="787" summary="" border="1">
<tbody>
<tr>
<td bgcolor="#cccccc"><strong> <font size="2">Total cell protein concentrations</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> Detergent soluble protein = 1-2 mg/10의 7제곱 mammalian<br />
cells or 100 -200 mg/ml for soluble proteins only</font></td>
</tr>
<tr>
<td><font size="2"> <strong>Specific protein concentrations</strong></font></td>
<td><font size="2"></font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><font size="2"> </font><strong><font size="2">Nucleus (200 um³):<br />
Abundant transcription factor<br />
Rare transcription factor</font></strong></td>
<td bgcolor="#cccccc"><font size="2"> 1nM (100,000 copies / nucleus)<br />
10 pM (1,000 copies / nucleus)</font></td>
</tr>
<tr>
<td><font size="2"> <strong>Serum</strong></font></td>
<td><font size="2"> 50 - 100 mg / ml</font></td>
</tr>
</tbody>
</table>