염석법

염석은 용해도가 큰 염을 단백질 혼합액에 가하여 
이온강도의 변화로 인해 단백질을 침전시키는 것이다.

 

                                                                                                                  황산암모늄 첨가 도표

		Final concentration of ammonium sulfate, % saturation
		10	20	25	30	33	35	40	45	50	55	60	65	70	75	80	90	100
		Grams solid ammonium sulfate to be added to 11. of solution
Initial concentration of ammonium sulfate,% saturation	0	56	114	144	176	196	209	243	277	313	351	390	430	472	516	561	662	767
	10		57	86	118	137	150	183	216	251	288	326	365	406	449	494	592	694
	20			29	39	78	91	123	155	189	225	262	300	340	382	424	520	619
	25				30	49	61	93	125	158	193	230	267	307	348	390	485	583
	30					19	30	62	94	127	162	198	235	273	314	356	449	546
	33						12	43	74	107	142	177	214	252	292	333	426	522
	35							31	63	94	129	164	200	238	278	319	411	506
	40								31	63	97	132	168	205	245	285	375	469
	45									62	65	99	134	171	210	250	339	431
	50										33	66	101	137	176	214	302	392
	55											33	67	103	141	179	264	353
	60												34	69	105	143	227	314
	65													34	70	107	190	275
	70														35	72	153	237
	75															36	115	198
	80																77	157
	90																	79

                                                                     -용액 1ℓ에 가할 고체상 황산암모늄의 g수