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Cellculture

404 bytes added, 20:32, 13 December 2006
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<palign="justify">&nbsp;사용용도</p>
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<palign="justify">pH조적은 배지의 중탄산 나트륨과 CO<sub>2</sub>가스의 평형에 의해 이루어진다.<br />
&nbsp; 5% CO<sub>2</sub> - 95% air 유지<br />
&nbsp; 재킷형 CO<sub>2 </sub>incubator 주로 사용 - water 재킷(멸균수) : 온도 조절<br />
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<palign="justify">&nbsp; 배양실&nbsp;:&nbsp; 무균실<br />
&nbsp; clean bench : 무균조작을 위한 무균상자,&nbsp;자외선 멸균장치 포함</p>
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<palign="justify">culture flask - 배양용량이 큰 배양용기, screw cap부착<br />
&nbsp; petri-dish - 조직배양용 dish(접착세포 배양), 일반세균 배양용dish(부유세포 배양)<br />
&nbsp; multi-well plate - 96 well 사용, 소량의 배양액</p>
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<palign="justify">&nbsp;&nbsp;온도조절</p>
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<palign="justify">&nbsp;&nbsp;무염색 세포관찰</p>
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<palign="justify">멸균장치</p>
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<palign="justify">&nbsp; 동결보존 -80도 유지</p>
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<palign="justify">세포와 배양액의 분리</p>
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<palign="justify">배지, 시약 등의 보존</p>
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<p align="center">[[cover slip]]</p>
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<td bordercolor="#ffffcc" width="762" bgcolor="#ffffcc" height="7"> <p align="justify">염색용</p> </td>
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<p>[[auto pipette]]</p>
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<td bordercolor="#ffffcc" width="762" bgcolor="#ffffcc" height="7"> <p align="justify">분주</p> </td>
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<td bordercolor="#66ffff" width="207" bgcolor="#66ffff" height="7">혈구 게시판</td>
<td bordercolor="#ffffcc" width="762" bgcolor="#ffffcc" height="7"> <p align="justify">세포수 측정</p> </td>
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<td bordercolor="#ffffcc" width="762" bgcolor="#ffffcc" height="51"> <p align="justify">&nbsp;&nbsp;culter counter<br />
&nbsp; 혈구 계산판<br />
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<p align="center">생세포수 계산</p>
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<td bordercolor="#ffffcc" width="762" bgcolor="#ffffcc" height="91"> <p align="justify">&nbsp; 원리 : 색소 배제 능력&nbsp;&nbsp; tryphan blue염색 - 사세포는 배제 능력이 없고 청색으로 염색한다.<br />
&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 생세포율(%)&nbsp; = (생세포수) / (생세포수 + 사세포수)<br />
&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;생세포수(cell / ml) = 전세포수(cells / ml) X 생존율(%) </p> </td>
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