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<font size="2"><font style="BACKGROUND-COLOR: #ccffcc"><strong><font size="4">Picrosirius Red Staining</font></strong><br /></font><br />
<strong>To make the Picrosirius stain</strong><br />
<br />
<br />
- Paraffin embedded, 5 or 10 um thick sections are stained as follows:<br />
<br />
Histoclear #1 - 3 minutes<br />
Histoclear #2 - 3 minutes<br />
<strong>Note : can add 0.2% phosphomolybdic acid in DD H2O for 2 minutes with quick rinse after 10 minute water step above.<br />
<br />
Note : In case offreezing section,</strong> freezing section, <br />
1) 60°C dry oven 30 min<br />
2) H2O rinse 10 min<br />
3) PS stain 90 min<br />
4) MQ washing </font>
