Difference between revisions of "Phi X 174"
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<ul> | <ul> | ||
| − | <li><a class="external text" title="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" href="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm | + | <li><a class="external text" title="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" rel="nofollow" href="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm">Description & Restriction Map: PhiX174 DNA</a> </li> |
</ul> | </ul> | ||
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| − | <div class="printfooter"></div> | + | <div class="printfooter"><br /> |
| + | Phi X 174 Genome sequence:<br /> | ||
| + | <br /> | ||
| + | <font face="Courier New"> 1 gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa<br /> | ||
| + | 61 aaattatctt gataaagcag gaattactac tgcttgttta cgaattaaat cgaagtggac<br /> | ||
| + | 121 tgctggcgga aaatgagaaa attcgaccta tccttgcgca gctcgagaag ctcttacttt<br /> | ||
| + | 181 gcgacctttc gccatcaact aacgattctg tcaaaaactg acgcgttgga tgaggagaag<br /> | ||
| + | 241 tggcttaata tgcttggcac gttcgtcaag gactggttta gatatgagtc acattttgtt<br /> | ||
| + | 301 catggtagag attctcttgt tgacatttta aaagagcgtg gattactatc tgagtccgat<br /> | ||
| + | 361 gctgttcaac cactaatagg taagaaatca tgagtcaagt tactgaacaa tccgtacgtt<br /> | ||
| + | 421 tccagaccgc tttggcctct attaagctca ttcaggcttc tgccgttttg gatttaaccg<br /> | ||
| + | 481 aagatgattt cgattttctg acgagtaaca aagtttggat tgctactgac cgctctcgtg<br /> | ||
| + | 541 ctcgtcgctg cgttgaggct tgcgtttatg gtacgctgga ctttgtggga taccctcgct<br /> | ||
| + | 601 ttcctgctcc tgttgagttt attgctgccg tcattgctta ttatgttcat cccgtcaaca<br /> | ||
| + | 661 ttcaaacggc ctgtctcatc atggaaggcg ctgaatttac ggaaaacatt attaatggcg<br /> | ||
| + | 721 tcgagcgtcc ggttaaagcc gctgaattgt tcgcgtttac cttgcgtgta cgcgcaggaa<br /> | ||
| + | 781 acactgacgt tcttactgac gcagaagaaa acgtgcgtca aaaattacgt gcggaaggag<br /> | ||
| + | 841 tgatgtaatg tctaaaggta aaaaacgttc tggcgctcgc cctggtcgtc cgcagccgtt<br /> | ||
| + | 901 gcgaggtact aaaggcaagc gtaaaggcgc tcgtctttgg tatgtaggtg gtcaacaatt<br /> | ||
| + | 961 ttaattgcag gggcttcggc cccttacttg aggataaatt atgtctaata ttcaaactgg<br /> | ||
| + | 1021 cgccgagcgt atgccgcatg acctttccca tcttggcttc cttgctggtc agattggtcg<br /> | ||
| + | 1081 tcttattacc atttcaacta ctccggttat cgctggcgac tccttcgaga tggacgccgt<br /> | ||
| + | 1141 tggcgctctc cgtctttctc cattgcgtcg tggccttgct attgactcta ctgtagacat<br /> | ||
| + | 1201 ttttactttt tatgtccctc atcgtcacgt ttatggtgaa cagtggatta agttcatgaa<br /> | ||
| + | 1261 ggatggtgtt aatgccactc ctctcccgac tgttaacact actggttata ttgaccatgc<br /> | ||
| + | 1321 cgcttttctt ggcacgatta accctgatac caataaaatc cctaagcatt tgtttcaggg<br /> | ||
| + | 1381 ttatttgaat atctataaca actattttaa agcgccgtgg atgcctgacc gtaccgaggc<br /> | ||
| + | 1441 taaccctaat gagcttaatc aagatgatgc tcgttatggt ttccgttgct gccatctcaa<br /> | ||
| + | 1501 aaacatttgg actgctccgc ttcctcctga gactgagctt tctcgccaaa tgacgacttc<br /> | ||
| + | 1561 taccacatct attgacatta tgggtctgca agctgcttat gctaatttgc atactgacca<br /> | ||
| + | 1621 agaacgtgat tacttcatgc agcgttacca tgatgttatt tcttcatttg gaggtaaaac<br /> | ||
| + | 1681 ctcttatgac gctgacaacc gtcctttact tgtcatgcgc tctaatctct gggcatctgg<br /> | ||
| + | 1741 ctatgatgtt gatggaactg accaaacgtc gttaggccag ttttctggtc gtgttcaaca<br /> | ||
| + | 1801 gacctataaa cattctgtgc cgcgtttctt tgttcctgag catggcacta tgtttactct<br /> | ||
| + | 1861 tgcgcttgtt cgttttccgc ctactgcgac taaagagatt cagtacctta acgctaaagg<br /> | ||
| + | 1921 tgctttgact tataccgata ttgctggcga ccctgttttg tatggcaact tgccgccgcg<br /> | ||
| + | 1981 tgaaatttct atgaaggatg ttttccgttc tggtgattcg tctaagaagt ttaagattgc<br /> | ||
| + | 2041 tgagggtcag tggtatcgtt atgcgccttc gtatgtttct cctgcttatc accttcttga<br /> | ||
| + | 2101 aggcttccca ttcattcagg aaccgccttc tggtgatttg caagaacgcg tacttattcg<br /> | ||
| + | 2161 ccaccatgat tatgaccagt gtttccagtc cgttcagttg ttgcagtgga atagtcaggt<br /> | ||
| + | 2221 taaatttaat gtgaccgttt atcgcaatct gccgaccact cgcgattcaa tcatgacttc<br /> | ||
| + | 2281 gtgataaaag attgagtgtg aggttataac gccgaagcgg taaaaatttt aatttttgcc<br /> | ||
| + | 2341 gctgaggggt tgaccaagcg aagcgcggta ggttttctgc ttaggagttt aatcatgttt<br /> | ||
| + | 2401 cagactttta tttctcgcca taattcaaac tttttttctg ataagctggt tctcacttct<br /> | ||
| + | 2461 gttactccag cttcttcggc acctgtttta cagacaccta aagctacatc gtcaacgtta<br /> | ||
| + | 2521 tattttgata gtttgacggt taatgctggt aatggtggtt ttcttcattg cattcagatg<br /> | ||
| + | 2581 gatacatctg tcaacgccgc taatcaggtt gtttctgttg gtgctgatat tgcttttgat<br /> | ||
| + | 2641 gccgacccta aattttttgc ctgtttggtt cgctttgagt cttcttcggt tccgactacc<br /> | ||
| + | 2701 ctcccgactg cctatgatgt ttatcctttg aatggtcgcc atgatggtgg ttattatacc<br /> | ||
| + | 2761 gtcaaggact gtgtgactat tgacgtcctt ccccgtacgc cgggcaataa cgtttatgtt<br /> | ||
| + | 2821 ggtttcatgg tttggtctaa ctttaccgct actaaatgcc gcggattggt ttcgctgaat<br /> | ||
| + | 2881 caggttatta aagagattat ttgtctccag ccacttaagt gaggtgattt atgtttggtg<br /> | ||
| + | 2941 ctattgctgg cggtattgct tctgctcttg ctggtggcgc catgtctaaa ttgtttggag<br /> | ||
| + | 3001 gcggtcaaaa agccgcctcc ggtggcattc aaggtgatgt gcttgctacc gataacaata<br /> | ||
| + | 3061 ctgtaggcat gggtgatgct ggtattaaat ctgccattca aggctctaat gttcctaacc<br /> | ||
| + | 3121 ctgatgaggc cgcccctagt tttgtttctg gtgctatggc taaagctggt aaaggacttc<br /> | ||
| + | 3181 ttgaaggtac gttgcaggct ggcacttctg ccgtttctga taagttgctt gatttggttg<br /> | ||
| + | 3241 gacttggtgg caagtctgcc gctgataaag gaaaggatac tcgtgattat cttgctgctg<br /> | ||
| + | 3301 catttcctga gcttaatgct tgggagcgtg ctggtgctga tgcttcctct gctggtatgg<br /> | ||
| + | 3361 ttgacgccgg atttgagaat caaaaagagc ttactaaaat gcaactggac aatcagaaag<br /> | ||
| + | 3421 agattgccga gatgcaaaat gagactcaaa aagagattgc tggcattcag tcggcgactt<br /> | ||
| + | 3481 cacgccagaa tacgaaagac caggtatatg cacaaaatga gatgcttgct tatcaacaga<br /> | ||
| + | 3541 aggagtctac tgctcgcgtt gcgtctatta tggaaaacac caatctttcc aagcaacagc<br /> | ||
| + | 3601 aggtttccga gattatgcgc caaatgctta ctcaagctca aacggctggt cagtatttta<br /> | ||
| + | 3661 ccaatgacca aatcaaagaa atgactcgca aggttagtgc tgaggttgac ttagttcatc<br /> | ||
| + | 3721 agcaaacgca gaatcagcgg tatggctctt ctcatattgg cgctactgca aaggatattt<br /> | ||
| + | 3781 ctaatgtcgt cactgatgct gcttctggtg tggttgatat ttttcatggt attgataaag<br /> | ||
| + | 3841 ctgttgccga tacttggaac aatttctgga aagacggtaa agctgatggt attggctcta<br /> | ||
| + | 3901 atttgtctag gaaataaccg tcaggattga caccctccca attgtatgtt ttcatgcctc<br /> | ||
| + | 3961 caaatcttgg aggctttttt atggttcgtt cttattaccc ttctgaatgt cacgctgatt<br /> | ||
| + | 4021 attttgactt tgagcgtatc gaggctctta aacctgctat tgaggcttgt ggcatttcta<br /> | ||
| + | 4081 ctctttctca atccccaatg cttggcttcc ataagcagat ggataaccgc atcaagctct<br /> | ||
| + | 4141 tggaagagat tctgtctttt cgtatgcagg gcgttgagtt cgataatggt gatatgtatg<br /> | ||
| + | 4201 ttgacggcca taaggctgct tctgacgttc gtgatgagtt tgtatctgtt actgagaagt<br /> | ||
| + | 4261 taatggatga attggcacaa tgctacaatg tgctccccca acttgatatt aataacacta<br /> | ||
| + | 4321 tagaccaccg ccccgaaggg gacgaaaaat ggtttttaga gaacgagaag acggttacgc<br /> | ||
| + | 4381 agttttgccg caagctggct gctgaacgcc ctcttaagga tattcgcgat gagtataatt<br /> | ||
| + | 4441 accccaaaaa gaaaggtatt aaggatgagt gttcaagatt gctggaggcc tccactatga<br /> | ||
| + | 4501 aatcgcgtag aggctttgct attcagcgtt tgatgaatgc aatgcgacag gctcatgctg<br /> | ||
| + | 4561 atggttggtt tatcgttttt gacactctca cgttggctga cgaccgatta gaggcgtttt<br /> | ||
| + | 4621 atgataatcc caatgctttg cgtgactatt ttcgtgatat tggtcgtatg gttcttgctg<br /> | ||
| + | 4681 ccgagggtcg caaggctaat gattcacacg ccgactgcta tcagtatttt tgtgtgcctg<br /> | ||
| + | 4741 agtatggtac agctaatggc cgtcttcatt tccatgcggt gcactttatg cggacacttc<br /> | ||
| + | 4801 ctacaggtag cgttgaccct aattttggtc gtcgggtacg caatcgccgc cagttaaata<br /> | ||
| + | 4861 gcttgcaaaa tacgtggcct tatggttaca gtatgcccat cgcagttcgc tacacgcagg<br /> | ||
| + | 4921 acgctttttc acgttctggt tggttgtggc ctgttgatgc taaaggtgag ccgcttaaag<br /> | ||
| + | 4981 ctaccagtta tatggctgtt ggtttctatg tggctaaata cgttaacaaa aagtcagata<br /> | ||
| + | 5041 tggaccttgc tgctaaaggt ctaggagcta aagaatggaa caactcacta aaaaccaagc<br /> | ||
| + | 5101 tgtcgctact tcccaagaag ctgttcagaa tcagaatgag ccgcaacttc gggatgaaaa<br /> | ||
| + | 5161 tgctcacaat gacaaatctg tccacggagt gcttaatcca acttaccaag ctgggttacg<br /> | ||
| + | 5221 acgcgacgcc gttcaaccag atattgaagc agaacgcaaa aagagagatg agattgaggc<br /> | ||
| + | 5281 tgggaaaagt tactgtagcc gacgttttgg cggcgcaacc tgtgacgaca aatctgctca<br /> | ||
| + | 5341 aatttatgcg cgcttcgata aaaatgattg gcgtatccaa cctgca<br /> | ||
| + | </font></div> | ||
Revision as of 20:37, 22 August 2008
The phi X 174 (or phi X) bacteriophage was the first organism to have its DNA-based genome sequenced, by Fred Sanger and his team in 1977.[1] In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.[2]
This phage has a very small amount of DNA. It has 11 genes in 5386 bases (it is single stranded) in a circular topology. Several of them expressing similar function in two groups. The GC-content is 44% and 95% of nucleotides are coding genes.
In 2003 it was reported that the whole genome of phi X 174 had been assembled synthetically from scratch.[3]
See also
- Bacteriophage MS2
References
- ^ Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M., "Nucleotide sequence of bacteriophage phi X174 DNA", Nature. 1977 Feb 24;265(5596):687-95
- ^ Fiers, W., and R. L. Sinsheimer, "The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure", J. Mol. Biol. 5:424-434, 1962
- ^ Smith, Hamilton O.; Clyde A. Hutchison, Cynthia Pfannkoch, J. Craig Venter (2003-12-23). "Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences 100 (26): 15440-15445. doi:. Retrieved on 2007-10-08.
