Difference between revisions of "Polony"
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<p>Polony is a contraction of "polymerase colony," a small colony of DNA.</p> | <p>Polony is a contraction of "polymerase colony," a small colony of DNA.</p> | ||
<p>Polonies are discrete clonal amplifications of a single DNA molecule, grown in a gel matrix. This approach greatly improves the signal-to-noise ratio. Polonies can be generated using several techniques that include solid-phase PCR in polyacrylamide gels. However, other earlier patented technologies, such as that from Manteia (acquired by Solexa), which generate DNA on a solid phase surface by bridge amplification - are generally referred to as "Clusters". The terminology and distinction between 'polony' and 'cluster' have become confused recently. Growth of clonal copies of DNA on bead surfaces remains to be generically named although some also seek to name this technique as a "polony" method.</p> | <p>Polonies are discrete clonal amplifications of a single DNA molecule, grown in a gel matrix. This approach greatly improves the signal-to-noise ratio. Polonies can be generated using several techniques that include solid-phase PCR in polyacrylamide gels. However, other earlier patented technologies, such as that from Manteia (acquired by Solexa), which generate DNA on a solid phase surface by bridge amplification - are generally referred to as "Clusters". The terminology and distinction between 'polony' and 'cluster' have become confused recently. Growth of clonal copies of DNA on bead surfaces remains to be generically named although some also seek to name this technique as a "polony" method.</p> | ||
| − | <p> | + | <p> </p> |
<p>In one conceptually simple way to create clusters, a solution containing DNA fragments is poured onto a microscope slide dilute enough so that individual molecules are separated. DNA polymerase is added, which copies each fragment repeatedly, creating millions of clusters, each of which contains only copies of the original fragment of DNA. These "clusters" are then used for various kinds of DNA research like DNA sequencing.</p> | <p>In one conceptually simple way to create clusters, a solution containing DNA fragments is poured onto a microscope slide dilute enough so that individual molecules are separated. DNA polymerase is added, which copies each fragment repeatedly, creating millions of clusters, each of which contains only copies of the original fragment of DNA. These "clusters" are then used for various kinds of DNA research like DNA sequencing.</p> | ||
<p>However, notwithstanding the erudition above, 'polony' also appears as a British slang term in Graham Greene's book 'Brighton Rock', meaning a plump person, likened to a fat sausage or 'Bologna'. It is also a type of finely processed pork that comes in a sausage shaped wrapping (pork bolony perhaps?).</p> | <p>However, notwithstanding the erudition above, 'polony' also appears as a British slang term in Graham Greene's book 'Brighton Rock', meaning a plump person, likened to a fat sausage or 'Bologna'. It is also a type of finely processed pork that comes in a sausage shaped wrapping (pork bolony perhaps?).</p> | ||
| − | <p><span class="mw-headline">References</span></p> | + | <p><span class="mw-headline"><strong><br /> |
| + | <font size="4">References</font></strong></span></p> | ||
<ul> | <ul> | ||
<li>Polony technology guide </li> | <li>Polony technology guide </li> | ||
<li>Patent (1999) : WO00/18957 </li> | <li>Patent (1999) : WO00/18957 </li> | ||
<li>Adessi C, Matton G, Ayala G, Turcatti G, Mermod J-J, Mayer P, Kawashima E. Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. (2000) 28:e87/1–e87/8. </li> | <li>Adessi C, Matton G, Ayala G, Turcatti G, Mermod J-J, Mayer P, Kawashima E. Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. (2000) 28:e87/1–e87/8. </li> | ||
| − | <li><a class="external text" title="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16847463" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16847463 | + | <li><a class="external text" title="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16847463" rel="nofollow" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=16847463">Fan Jb, Chee, MS, Gunderson, KL (2006) Highly parallel genomic assays. Nature Reviews of Genetics (8):632-44.</a> </li> |
| − | <li><a class="external text" title="http://www.sciencemag.org/cgi/content/abstract/1117389" href="http://www.sciencemag.org/cgi/content/abstract/1117389 | + | <li><a class="external text" title="http://www.sciencemag.org/cgi/content/abstract/1117389" rel="nofollow" href="http://www.sciencemag.org/cgi/content/abstract/1117389">Shendure, Porreca et al. Accurate multiplex polony sequencing of an evolved bacterial genome</a> </li> |
| − | <li><a class="external text" title="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=hulib&db=pubmed&cmd=Retrieve&list_uids=16732271" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=hulib&db=pubmed&cmd=Retrieve&list_uids=16732271 | + | <li><a class="external text" title="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=hulib&db=pubmed&cmd=Retrieve&list_uids=16732271" rel="nofollow" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?holding=hulib&db=pubmed&cmd=Retrieve&list_uids=16732271"><font color="#810081">Zhang et al. Sequencing genomes from single cells by polymerase cloning.</font></a> </li> |
</ul> | </ul> | ||
| − | <p><br /> | + | <p><strong><br /> |
| + | <font size="4">External links</font><br /> | ||
| + | </strong>[http://genomics.org Genomics.org]<br /> | ||
| + | [http://omics.org Omics.org]<br /> | ||
| + | <br /> | ||
<br /> | <br /> | ||
</p> | </p> | ||
Latest revision as of 17:48, 24 May 2008
Polony is a contraction of "polymerase colony," a small colony of DNA.
Polonies are discrete clonal amplifications of a single DNA molecule, grown in a gel matrix. This approach greatly improves the signal-to-noise ratio. Polonies can be generated using several techniques that include solid-phase PCR in polyacrylamide gels. However, other earlier patented technologies, such as that from Manteia (acquired by Solexa), which generate DNA on a solid phase surface by bridge amplification - are generally referred to as "Clusters". The terminology and distinction between 'polony' and 'cluster' have become confused recently. Growth of clonal copies of DNA on bead surfaces remains to be generically named although some also seek to name this technique as a "polony" method.
In one conceptually simple way to create clusters, a solution containing DNA fragments is poured onto a microscope slide dilute enough so that individual molecules are separated. DNA polymerase is added, which copies each fragment repeatedly, creating millions of clusters, each of which contains only copies of the original fragment of DNA. These "clusters" are then used for various kinds of DNA research like DNA sequencing.
However, notwithstanding the erudition above, 'polony' also appears as a British slang term in Graham Greene's book 'Brighton Rock', meaning a plump person, likened to a fat sausage or 'Bologna'. It is also a type of finely processed pork that comes in a sausage shaped wrapping (pork bolony perhaps?).
References
- Polony technology guide
- Patent (1999) : WO00/18957
- Adessi C, Matton G, Ayala G, Turcatti G, Mermod J-J, Mayer P, Kawashima E. Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms. Nucleic Acids Res. (2000) 28:e87/1–e87/8.
- Fan Jb, Chee, MS, Gunderson, KL (2006) Highly parallel genomic assays. Nature Reviews of Genetics (8):632-44.
- Shendure, Porreca et al. Accurate multiplex polony sequencing of an evolved bacterial genome
- Zhang et al. Sequencing genomes from single cells by polymerase cloning.
External links
Genomics.org
Omics.org
