Difference between revisions of "PCR"

Latest revision as of 12:36, 22 February 2008

Polymerase Chain Reaction(PCR)

피씨알

@@ Line 1: / Line 1: @@
-<span style="LINE-HEIGHT: 160%"><font size="3"><font face="Times New Roman"><strong>[[Polymerase Chain Reaction(PCR)]]<br /></strong><br />&nbsp;</font></font><font size="3"><font face="Times New Roman"><strong>PCR의 원리 <br /></strong>Polymerase chain reaction(PCR)은 1983년 Mullis와 Faloona가 처음으로 개발하였다. 시험관에서의 DNA 증폭 방법으로, PCR은 DNA 중합효소에 의한 DNA 복제 특징을 이용한 것으로 유전자를 분석하고 연구하는데 있어서 가장 큰 문제점은 복잡한 전체 genome중에 연구하고자 하는 유전자가 희귀한데, PCR은 특정 DNA Sequence의 copy수를 기하급수적으로 증폭시키는 것이 가능하다. 따라서&nbsp;유전자 클로닝을 포함한 분자유전학, 의학생물학, 집단유전학등 생명과학의 전 분야에 혁신을 일으킬 수 있었다.<br /><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">중합효소</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">연쇄반응</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> (polymerase chain reaction, PCR)</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">은</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">특정</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> DNA </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">부위를</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">특이적으로</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">반복</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">합성하여</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">시험관내에서</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">원하는</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> DNA </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">분자를</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">증폭시키는</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">방법으로서</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">, </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">아주</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">적은</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">양의</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">DNA</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">를</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">이용하여</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">많은</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">양의</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> DNA </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">합성이</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">가능하므로</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">분자</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">생물학적으로</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">제한효소의</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">발견만큼</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">획기적인</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">것이라고</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">할</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">수</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">있다</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">. </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">즉</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> genomic DNA</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">와</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">같은</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">아주</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">큰</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> DNA</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">로부터</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">원하는</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> DNA </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">부분만을</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">선택적으로</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">증폭시킨</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">후</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">일반적으로</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">사용되는</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> agarose gel</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">이나</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> polyacrylamide gel </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">상에서</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">뚜렷하게</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">보이는</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> band</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">로</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">가시화할</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">수가</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">있다</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">. </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">중합효소</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">연쇄반응을</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">통하여</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> in vitro</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">상에서</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">특정</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">부위의</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> DNA</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">를</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> 105</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">～</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">108</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">배까지</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">수</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">시간</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">내에</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">증폭시킬</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">수</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">있다</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">, </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">이렇게</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">증폭된</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> DNA</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">는</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">여러</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">실험에</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">이용하며</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">, </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">실험</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">결과를</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">토대로</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">의학적인</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">연구에</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">응용할</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">수가</span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕"> </span><span style="FONT-SIZE: 10pt; FONT-FAMILY: 바탕; mso-bidi-font-size: 12.0pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA">있다</span><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: 'Times New Roman'; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: KO; mso-bidi-language: AR-SA; mso-fareast-font-family: 바탕">.</span><br />특히 PCR에 사용하는 DNA 중합효소인 Taq polymerase는 고온의 온천수(72-74℃)에서 서식하는 미생물에서 분리한 것으로 72℃에서 활성이 가장 높으며 95℃ 이상의 고온에서도 매우 안정하여 PCR을 자동화시키는데 결정적인 역할을 하게 되었다. <br />PCR의 원리는 DNA의 양쪽 가닥을 주형으로 하여 원하는 DNA를 증폭시키는 방법으로 전체 DNA로부터 원하는 DNA 특정 부분을 선택적으로 증폭시.<br />DNA polymerase가 DNA합성 시작을 위해서는 외가닥 DNA의 주형이 있어야 하므로, double stand(이중가닥)으로 되어있는 주형을 고온(95℃)에서 denaturation(변성)을 시켜야 한다.<br />변성된 single stand DNA의 양 끝에 상보적으로 결합할 수 있는 작은 DNA 조각(primer)을 넣어주어 낮은 온도(50-65℃)에서 annealing(결합).<br />결합한 후에 72-74 ℃에서 Taq polymerase가 DNA 합성을 한다. 이렇게 'denaturation(변성)-annealing(결합)-extension(신장)'의 cycle이 30회 정도 반복 수행되어 DNA를 증폭킨다. 따라서 이론적으로 한 가닥의 주형으로 30회 cycle을 수행하면 230의 DNA 가닥을 얻을 수 있다. <br /><br /></font></font>
+<p>[[Polymerase Chain Reaction(PCR)]]</p>
-<p><font size="3"><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 13pt; FONT-FAMILY: 'Times New Roman'">* PCR </span></strong><font face="Times New Roman"><strong style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 13pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 13pt; FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-SIZE: 13pt; mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">원리</span></strong><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-SIZE: 13pt; FONT-FAMILY: 'Times New Roman'">
+<p>[[피씨알]]<br />
-<p>&nbsp;</p>
+</p>
-</span></strong></font></font></p>
-<p><font size="3"><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">중합효소</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">연쇄반응은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">다음의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">세</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">단계로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이루어진다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">1) DNA</span></strong><font face="Times New Roman"><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">변성</span></strong><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">(denaturation)
-<p>&nbsp;</p>
-</span></strong></font></font></p>
-<p style="TEXT-INDENT: 12pt; mso-char-indent-count: 1.0"><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">90</span><font face="Times New Roman"><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">℃</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">96</span><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">℃</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가열하여</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> double strand DNA(ds DNA)</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">를</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> single strand DNA(ssDNA)</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">분리시킨다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">높은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">온도일수록</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> ssDNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">잘</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이행되지만</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> Taq DNA polymerase</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">도</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">온도가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">아주</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">높은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">상태에서는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">활성도가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">낮아질</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있으므로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">보통</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 94</span><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">℃</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">쓴다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. Cycle</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">처음은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">확실한</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">변성을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">위하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">약</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 5</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">분간</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시간을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">주어야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">2) Primer</span></strong><font face="Times New Roman"><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">결합</span></strong><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">(annealing)
-<p>&nbsp;</p>
-</span></strong></font></font></p>
-<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">&nbsp;50</span><font face="Times New Roman"><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">℃</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">65</span><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">℃</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">진행되며</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">, </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">염기간에</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> G</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">와</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> C</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">세</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">군데에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수소결합이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">일어나고</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">, A</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">와</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> T</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">두</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">군데에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">결합이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">일어나므로</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> G+C </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비율에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">따라</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">결합온도에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">변화를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">주어야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비특이</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> PCR </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">산물이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">형성되는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">경우</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">결합온도를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">올려서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응시켜</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">보면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비특이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">산물을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">줄이는데</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">도움이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">되는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">3) DNA</span></strong><font face="Times New Roman"><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성</span></strong><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">(polymerization)
-<p>&nbsp;</p>
-</span></strong></font></font></p>
-<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">&nbsp;70</span><font face="Times New Roman"><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">℃</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">74</span><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">℃</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시행하며</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">원하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> PCR </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">산물의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">크기가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">크거나</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응요소의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">농도가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">낮을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">때에는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시간을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">연장시키는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">좋다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. Taq DNA polymerase</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">보통</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 1</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">분에</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 2,000</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">4,000 nucleotides</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성할</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있으므로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">원하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> PCR </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">산물의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">크기</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 1 kb</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">마다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 1</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">분</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정도의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시간을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">배당하면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">충분히</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">일어날</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. Cycle</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">계속되면서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">효소</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">활성이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">감소할</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있고</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">산물은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">점점</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">많이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">존재하게</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">되므로</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> cycle </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">후반부에는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응시간을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">조금씩</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">늘려</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것도</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">좋은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">방법의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">하나이며</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">, </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">마지막</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> cycle</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">에는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시간을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">충분히</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> (10</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">분</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">) </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">주어</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">효소의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">활성이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">충분히</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">발휘되도록</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">&nbsp;</span><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">위</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">과정이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">계속</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반복하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">진행되면서</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">(</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">보통</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 25</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">35</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">회</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">) </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">원하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">부분을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">증폭시키는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">중합효소</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">연쇄반응의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">원리이다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<font size="3"><font face="Times New Roman"><strong>2. PCR에 필요한 재료 <br /></strong>- 주형 DNA: 증폭 대상이 되는 DNA . <br />-&nbsp;primer: DNA 중합효소가 DNA 합성을 개시하게 하는 짧은 외가닥 DNA. 증폭할 부분을 결정. <br />- dNTPs (dATP, dCTP, dGTP, dTTP): DNA를 합성하는 재료가 되는&nbsp;뉴클레오티드 염기. <br />- [[Taq polymerase]]: 열에 특별히 강한 DNA 합성효소.&nbsp;<br /><br /></font></font>
-<p><font size="3"><font face="Times New Roman"><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="mso-bidi-font-family: 'Times New Roman'">※</span></strong><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> PCR </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">각</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">성분에</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">대해</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">고려할</span></strong><strong style="mso-bidi-font-weight: normal"><span style="FONT-FAMILY: 'Times New Roman'"> </span></strong><strong style="mso-bidi-font-weight: normal"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">점</span></strong><strong style="mso-bidi-font-weight: normal"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">
-<p>&nbsp;</p>
-</span></strong></font></font></p>
-<p><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"><font size="3">&nbsp;1. Template DNA
-<p>&nbsp;</p>
-</font></span></p>
-<p><font size="3"><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">선택하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">증폭하고자</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> target DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">단편을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가리키는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">용어로서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">세포내의</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> chromosomal DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">나</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">전염성</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">병원체의</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">또는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> RNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">로부터</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">제조한</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> complementary DNA (cDNA), plasmid DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">등을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">쓸</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">불순물이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">섞여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있으면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">억제할</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있으므로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가능한</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정제하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">사용하는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">좋으며</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">, </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정제가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">안</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">된</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">경우에는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">적을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">때</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">충분히</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">일어나지</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">않고</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">많으면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비특이적</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">일어나므로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">주의해야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">Chromosomal DNA</span><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">인</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">경우</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 1 </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">㎍</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이내</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">, plasmid DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">경우</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 100 pg</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">100 ng </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정도를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">일반적으로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">사용하지만</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">사용하고자</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">내에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">증폭하고자</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> target DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> copy </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">따라</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">달라질</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">전기영동</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">상에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비특이</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">나타나거나</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">끌린</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">모양이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">나타나면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">맨</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">먼저</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> template DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">줄이는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시도하는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">좋다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"><font size="3">&nbsp;2. Primer
-<p>&nbsp;</p>
-</font></span></p>
-<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">Template DNA </span><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가운데</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">선택적으로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">증폭시키고자</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">하는</span><span style="FONT-FAMILY: 'Times New Roman'"> <span lang="EN-US">target DNA </span></span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">단편의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양쪽</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">끝에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">안쪽으로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">약</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 20 bp </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">내외의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">염기서열에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">대응하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> oligonucleotide</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">를</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성기로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이용한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. G+C </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비율이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반정도</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">되게</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">디자인하는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것이</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> primer</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">와</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> template </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">간의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">결합력을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">강하게</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">유지하는데</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">도움이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">되며</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> G+C </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">함량이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">많을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">경우에는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> annealing </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">온도를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">높이면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">되지만</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">, </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">무엇보다도</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양쪽</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> primer</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> annealing </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">온도를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비슷하게</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">만들어야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">또한</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양쪽</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> primer</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">서로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">상보적인</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">염기</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">결합이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">일어나지</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">않도록</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">하고</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가능하면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응산물</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">내에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">염기서열을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">분석하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비특이</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">생성되지</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">않도록</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">고안해야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. Primer</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">디자인이</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> PCR </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">성공</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">유무의</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 90%</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">차지한다고</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">할</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있기</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">때문에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성하기</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">전에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">신중히</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">고려해야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">할</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">것이다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. Primer </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">염기서열을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">적어</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">바이오니아</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">등의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">회사에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의뢰하면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">주문에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">따라서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정제도</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">해준다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가능하면</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> PAGE </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정제된</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> primer</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">쓰도록</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. Primer</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양은</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 0.1</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">0.5 &mu;M</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시행한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"><font size="3">&nbsp;3. dNTP (deoxynucleotide triphosphate)
-<p>&nbsp;</p>
-</font></span></p>
-<p><font size="3"><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시약</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">상에서</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> dATP, dCTP, dGTP</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">와</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> dTTP(</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">각각</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 100 mM stock)</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">구입한</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">후</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">혼합하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">사용한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양은</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> target DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">길이에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">따라</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">다를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">수</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있으나</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">각각</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 20</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">200 &mu;M </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정도를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">사용한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">&nbsp;4. 10x PCR </span><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">완충용액</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">(buffer)
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"><font size="3">&nbsp;5. Taq DNA polymerase
-<p>&nbsp;</p>
-</font></span></p>
-<p style="TEXT-INDENT: 6pt; mso-char-indent-count: .5"><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">Taq</span><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">온천에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">사는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> Thermus aquaticus</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">라는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">균주에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">따온</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이름이다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">균의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">활동</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">적정온도는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 70&deg;C</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">～</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">74&deg;C</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이며</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 95&deg;C</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">에서</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 40</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">분이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">지나도</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 50% </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정도의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">활성은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">남아</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">있다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">효소가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">발견되기</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">전에는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> Klenow </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">효소를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이용하여</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">매</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> cycle</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">마다</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">효소를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">첨가해</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">주면서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">시켜야만</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">했다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">DNA </span><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">오차율은</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 1.1X10-4 bp</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">로</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA polymerase I</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">오차율</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> (1.0X10-5)</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">보다</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">높는데</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> Taq DNA polymerase</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">잘못</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">들어간</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> nucleotide</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">를</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">삭제하고</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">다시</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">합성하는</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> proofreading </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">기능</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> (3'&rarr;5' exonuclease activity)</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">없기</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">때문이다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">. </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">따라서</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> PCR </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">산물에서</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">유전자의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">변이가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">발견되더라도</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반드시</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">확인과정을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">거쳐야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<p><font size="3"><font face="Times New Roman"><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한번의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">반응에</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">사용하는</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">효소의</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양은</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">보통</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> 0.5-5 unit </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">정도인데</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">양이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">너무</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">많으면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">비특이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">적인</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'"> DNA</span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">형성될</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">가능성이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">많고</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">, </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">너무</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">적으면</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">증폭</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">산물이</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">부족하게</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">되므로</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">최적</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">조건을</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">찾도록</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">노력해야</span><span style="FONT-FAMILY: 'Times New Roman'"> </span><span style="mso-ascii-font-family: 'Times New Roman'; mso-hansi-font-family: 'Times New Roman'; mso-bidi-font-family: 'Times New Roman'">한다</span><span lang="EN-US" style="FONT-FAMILY: 'Times New Roman'">.
-<p>&nbsp;</p>
-</span></font></font></p>
-<br /><font size="3"><font face="Times New Roman"><strong>3. PCR의 응용분야 <br /></strong>- 유전자 지도의 작성, Human [[Genome]] Project(인간 유전체 연구)에서 염기 서열 분석시 사용을 한다. <br />- 멸종된 과거 생물이나 현존 생물의 상호 유연관계를 규명하는 유전적 구성을 분석하는 진화 생물학이용에 이용이 가능하다. <br />- 친자확인, 범인 식별 등 [[Genetic Finger Printing]](유전적 지문 채취)을 이용한 법의학 분야에 활용이 가능하다. <br />- [[돌연변이]]분석, [[유전병]]진단, [[cDNA]] 합성 등에 이용에 쓰일 수 있다. <br />- 세포로부터 한 특정 DNA 조각을 직접 클로닝하는데 쓰일 수 있다.&nbsp;<br />- 바이러스나 각종 병원균 검출 초기식별에 사용이 가능하다. <br /></font></font></span>

Difference between revisions of "PCR"

Latest revision as of 12:36, 22 February 2008

Navigation menu

Views

Personal tools

Related Links

Advertisements

Search

Tools

Related Links[Edit]