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구체적인 단백질 실험 프로토콜

7,746 bytes added, 03:35, 19 February 2007
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<p>&nbsp;<br />
<br />
<strong><font size="3"><em>No9. David's life chart2<br />
</em></font></strong><br />
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</table>
</p>
<br />
<br />
<strong><em><font size="3">David's life chart 3<br />
</font></em></strong><hr />
&nbsp;<strong><font size="3">Some useful nucleotide dimensions</font></strong><hr />
<font size="2">&nbsp;</font><strong><font size="2">1cm of DNA &asymp;3 * 10의 6제곱 nucleotides<br />
</font></strong>
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<tbody>
<tr>
<td bgcolor="#0066ff"><strong><font size="2">&nbsp;<font color="#ffff99">Organism</font></font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2">&nbsp;Base pairs/<br />
&nbsp;haploid genome</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2">&nbsp;Base pairs/<br />
&nbsp;diploid genome</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2">&nbsp;Length / cell</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2">&nbsp;Mass</font></strong></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2">&nbsp;Human</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;3 * 10의 9제곱</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;6 * 10의 9제곱</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;2 meters (diploid)</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;6 pg</font></td>
</tr>
<tr>
<td><strong><font size="2">&nbsp;Fly</font></strong></td>
<td><font size="2">&nbsp;1.65 * 10의 8제곱</font></td>
<td><font size="2">&nbsp;3.3 * 10의 8제곱</font></td>
<td><font size="2">&nbsp;100 cm (diploid)</font></td>
<td><font size="2">&nbsp;0.3 pg</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2">&nbsp;Yeast</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;1.35 * 10의 7제곱</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;2.7 * 10의 7제곱</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;10 cm (diploid)</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;0.03 pg</font></td>
</tr>
<tr>
<td><strong><em><font size="2">&nbsp;E.coli</font></em></strong></td>
<td><font size="2">&nbsp;4.7 * 10의 6제곱</font></td>
<td><font size="2">&nbsp;-</font></td>
<td><font size="2">&nbsp;1.5 cm (haploid)</font></td>
<td><font size="2">&nbsp;0.0045 pg</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2">&nbsp;SV40</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;5 * 10의 3제곱</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;- </font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;1.7 nm</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;0.000006 pg</font></td>
</tr>
</tbody>
</table>
<br />
<br />
<br />
<strong><font size="3">Some useful cell dimensions<br />
<br />
</font></strong>
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<td bgcolor="#0066ff"><strong>&nbsp;<font color="#ffff99" size="2">Organism</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2">&nbsp;Dimensions</font></strong></td>
<td bgcolor="#0066ff"><strong><font color="#ffff99" size="2">&nbsp;Volume</font></strong></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><em><font size="2">&nbsp;S.cerevisiae</font></em></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;5 um</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;66 um&sup3;</font></td>
</tr>
<tr>
<td><strong><em><font size="2">&nbsp;S. pombe</font></em></strong></td>
<td><font size="2">&nbsp;2 * 7 um</font></td>
<td><font size="2">&nbsp;22 um&sup3;</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2">&nbsp;Mammalian cell</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;10-20 um</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;500-4,000 um&sup3;</font></td>
</tr>
<tr>
<td><strong><em><font size="2">&nbsp;E.coli</font></em></strong></td>
<td><font size="2">&nbsp;1 * 3 um</font></td>
<td><font size="2">&nbsp;2 um&sup3;</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2">&nbsp;Mammalian mitochondrion</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;1 um </font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;0.5 um&sup3;</font></td>
</tr>
<tr>
<td><strong><font size="2">&nbsp;Mammalian nucleus</font></strong></td>
<td><font size="2">&nbsp;5-10 um</font></td>
<td><font size="2">&nbsp;66-500 um&sup3;</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2">&nbsp;Plant chloroplast</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;1 * 4 um</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;3 um&sup3;&nbsp;</font></td>
</tr>
<tr>
<td><strong><font size="2">&nbsp;Bacteriophage lambda</font></strong></td>
<td><font size="2">&nbsp;50 nm (head only)</font></td>
<td><font size="2">&nbsp;6.6 * 10의 -5제곱 um&sup3;</font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><strong><font size="2">&nbsp;Ribosome</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;30 nm diameter</font></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;1.4 * 10의 -5제곱 um&sup3;</font></td>
</tr>
<tr>
<td><strong><font size="2">&nbsp;Globular monomeric protein</font></strong></td>
<td><font size="2">&nbsp;5 nm diameter</font></td>
<td><font size="2">&nbsp;6.6 * 10의 -8제곱 um&sup3;</font></td>
</tr>
</tbody>
</table>
<br />
<br />
<br />
<strong><font size="3">Some useful concentrarions</font></strong><br />
<br />
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<td bgcolor="#cccccc"><strong>&nbsp;<font size="2">Total cell protein concentrations</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;Detergent soluble protein = 1-2 mg/10의 7제곱 mammalian<br />
&nbsp;cells or 100 -200 mg/ml for soluble proteins only</font></td>
</tr>
<tr>
<td><font size="2">&nbsp;<strong>Specific protein concentrations</strong></font></td>
<td><font size="2"></font></td>
</tr>
<tr>
<td bgcolor="#cccccc"><font size="2">&nbsp;</font><strong><font size="2">Nucleus (200 um&sup3;):<br />
&nbsp;Abundant transcription factor<br />
&nbsp;Rare transcription factor</font></strong></td>
<td bgcolor="#cccccc"><font size="2">&nbsp;1nM (100,000 copies / nucleus)<br />
&nbsp;10 pM (1,000 copies / nucleus)</font></td>
</tr>
<tr>
<td><font size="2">&nbsp;<strong>Serum</strong></font></td>
<td><font size="2">&nbsp;50 - 100 mg / ml</font></td>
</tr>
</tbody>
</table>
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