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<font size="2"><strong><font size="4">Picrosirius Red Staining</font></strong><br /><br /><strong>To make the Picrosirius stain</strong><br />
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- Make saturated aqueous picric acid : heat 2~3 heaping teaspoons picric acid in 500+ ml distilled water, then cool. We use a magnetic stirrer / hot plate. If yellow needle-like crystals are not present when cool, then temperature was not high enough, or more acid is needed. Try heating again. Note : dry picric acid not in solution is very dangerous (combustible), so keep wet! Drain liquid for stain ; crystals may be used again.<br />
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- 250 ml are needed for proper staining procedure.<br />
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<strong><br />Stainig :</strong> <br /><br />
- Paraffin embedded, 5 or 10 um thick sections are stained as follows:<br />
Histoclear #1 - 3 minutes<br />
Histoclear #6 - 3 minutes<br />
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<strong>Note : can add 0.2% phosphomolybdic acid in DD H2O for 2 minutes with quick rinse after 10 minute water step above.<br />
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Note : In case of</strong> freezing section, <br /> 1) 60°C dry oven 30 min<br /> 2) H2O rinse 10 min<br /> 3) PS stain 90 min<br /> 4) MQ washing </font>