http://Opengenome.net/index.php?action=history&feed=atom&title=Sepharose 2026-05-13T08:42:46Z Revision history for this page on the wiki MediaWiki 1.31.3 http://Opengenome.net/index.php?title=Sepharose&diff=29396&oldid=prev 2009-05-27T10:34:37Z

<p></p> <p><b>New page</b></p><div>&lt;p&gt;&lt;strong&gt;Sepharose&lt;/strong&gt; is a tradename for a crosslinked, beaded-form of &lt;span class=&quot;mw-redirect&quot;&gt;agarose&lt;/span&gt; (a polysaccharide polymer material extracted from seaweed). It is commonly used in affinity chromatography as the matrix. Protein A/G-agarose chromatography provides a simple and efficient method for purifying &lt;span class=&quot;mw-redirect&quot;&gt;antibodies&lt;/span&gt;, streptavidin-agarose is used to purify biotin-labeled molecules, etc.&lt;/p&gt;<br /> &lt;p&gt;Sepharose/agarose, combined with some form of activation chemistry, is also used to immobilize &lt;span class=&quot;mw-redirect&quot;&gt;enzymes&lt;/span&gt;, &lt;span class=&quot;mw-redirect&quot;&gt;antibodies&lt;/span&gt; and other &lt;span class=&quot;mw-redirect&quot;&gt;proteins&lt;/span&gt; and &lt;span class=&quot;mw-redirect&quot;&gt;peptides&lt;/span&gt; through &lt;span class=&quot;mw-redirect&quot;&gt;covalent&lt;/span&gt; attachment to the resin.&lt;span class=&quot;external autonumber&quot;&gt;&amp;nbsp; Common activation chemistries include cyanogen bromide (CNBr) activation and reductive amination of &lt;span class=&quot;mw-redirect&quot;&gt;aldehydes&lt;/span&gt; to attach proteins to the agarose resin through lysine side chains. Iodoacetyl functional groups can be added to selectively bind cysteine side chains and this method is often used to immobilize peptides.&lt;/span&gt;&lt;/p&gt;<br /> &lt;h2&gt;&lt;strong&gt;Common products&lt;/strong&gt;&lt;/h2&gt;<br /> &lt;p&gt;&lt;a href=&quot;javascript:void(0);/*1243420148883*/&quot;&gt;&lt;strong&gt;Blue Sepharose&amp;reg; CL-6B&lt;/strong&gt;&lt;/a&gt;&lt;/p&gt;<br /> &lt;p id=&quot;productName&quot; class=&quot;productGeneral&quot;&gt;&lt;a href=&quot;javascript:void(0);/*1243420367009*/&quot;&gt;&lt;strong&gt;Con-A sepharose&amp;reg; 4B&lt;/strong&gt;&lt;/a&gt;&lt;/p&gt;<br /> &lt;p id=&quot;productName&quot; class=&quot;productGeneral&quot;&gt;&lt;a href=&quot;javascript:void(0);/*1243420411259*/&quot;&gt;&lt;strong&gt;Glutathione Sepharose&amp;reg;&lt;/strong&gt;&lt;/a&gt;&lt;/p&gt;<br /> &lt;p id=&quot;productName&quot; class=&quot;productGeneral&quot;&gt;&lt;strong&gt;&lt;br /&gt;<br /> &lt;/strong&gt;&lt;/p&gt;</div>

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