Phi X 174 - Revision history

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WikiSysop at 11:39, 22 August 2008

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<p>The <strong>phi X 174</strong> (or <strong>phi X</strong>) bacteriophage was the first organism to have its DNA-based <font color="#810081">genome</font> sequenced, by Fred Sanger and his team in 1977.<sup class="reference" id="cite_ref-0">[1]</sup> In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.<sup class="reference" id="cite_ref-1">[2]</sup></p>
<p>This phage has a very small amount of DNA. It has 11 genes in 5386 bases (it is single stranded) in a circular topology. Several of them expressing similar function in two groups. The GC-content is 44% and 95% of nucleotides are coding genes.</p>
<p>In 2003 it was reported that the whole genome of phi X 174 had been assembled synthetically from scratch.<sup class="reference" id="cite_ref-2">[3]</sup></p>
<p> </p>
<h2><span class="mw-headline">See also</span></h2>
<ul>
<li>Bacteriophage MS2 </li>
</ul>
<p> </p>
<h2><span class="mw-headline">References</span></h2>
<ol class="references">
<li id="cite_note-0"><strong>^</strong> Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M., "Nucleotide sequence of bacteriophage phi X174 DNA", <em>Nature</em>. 1977 Feb 24;265(5596):687-95 </li>
<li id="cite_note-1"><strong>^</strong> Fiers, W., and R. L. Sinsheimer, "The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure", <em>J. Mol. Biol.</em> <strong>5:</strong>424-434, 1962 </li>
<li id="cite_note-2"><strong>^</strong> <cite style="FONT-STYLE: normal">Smith, Hamilton O.; Clyde A. Hutchison, Cynthia Pfannkoch, J. Craig Venter (2003-12-23). "Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides". <em>Proceedings of the National Academy of Sciences</em> <strong>100</strong> (26): 15440-15445. doi:<span class="neverexpand">10.1073/pnas.2237126100</span><span class="reference-accessdate">. Retrieved on 2007-10-08.</span></cite><span class="reference-accessdate"><span class="Z3988" title="ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Generating+a+synthetic+genome+by+whole+genome+assembly%3A+%7Bphi%7DX174+bacteriophage+from+synthetic+oligonucleotides&rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences&rft.date=2003-12-23&rft.volume=100&rft.issue=26&rft.aulast=Smith&rft.aufirst=Hamilton+O.&rft.pages=15440-15445&rft_id=info:doi/10.1073%2Fpnas.2237126100&rft_id=http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fcontent%2Fabstract%2F100%2F26%2F15440"><span style="DISPLAY: none"> </span></span></span> </li>
</ol>
<p> </p>
<h2><span class="mw-headline">External links</span></h2>
<ul>
<li><a class="external text" title="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" href="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" rel="nofollow">Description & Restriction Map: PhiX174 DNA</a> </li>
</ul>
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-<p>The <strong>phi X 174</strong> (or <strong>phi X</strong>) bacteriophage was the first organism to have its DNA-based <font color="#810081">genome</font> sequenced, by Fred Sanger and his team in 1977.<sup class="reference" id="cite_ref-0">[1]</sup> In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.<sup class="reference" id="cite_ref-1">[2]</sup></p>
+<p><font color="#000000">The <strong>phi X 174</strong> (or <strong>phi X</strong>) bacteriophage was the first organism to have its DNA-based genome sequenced, by Fred Sanger and his team in 1977.<sup class="reference" id="cite_ref-0">[1]</sup> In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.<sup class="reference" id="cite_ref-1">[2]</sup></font></p>
-<p>This phage has a very small amount of DNA. It has 11 genes in 5386 bases (it is single stranded) in a circular topology. Several of them expressing similar function in two groups. The GC-content is 44% and 95% of nucleotides are coding genes.</p>
+<p><font color="#000000">This phage has a very small amount of DNA. It has 11 genes in 5386 bases (it is single stranded) in a circular topology. Several of them expressing similar function in two groups. The GC-content is 44% and 95% of nucleotides are coding genes.</font></p>
 <p>In 2003 it was reported that the whole genome of phi X 174 had been assembled synthetically from scratch.<sup class="reference" id="cite_ref-2">[3]</sup></p>
 <p>&nbsp;</p>

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      <li><a class="external text" title="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" rel="nofollow" href="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm">Description &amp; Restriction Map: PhiX174 DNA</a> </li>
 </ul>
+<p><br />
+<strong><font size="5">Phi X 174 Genome sequence</font></strong></p>
 <font face="Courier New">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 1&nbsp; &nbsp;gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa<br />
 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 61&nbsp; aaattatctt gataaagcag gaattactac tgcttgttta cgaattaaat cgaagtggac<br />

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      <li><a class="external text" title="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" rel="nofollow" href="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm">Description &amp; Restriction Map: PhiX174 DNA</a> </li>
 </ul>
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 <font face="Courier New">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 1&nbsp; &nbsp;gagttttatc gcttccatga cgcagaagtt aacactttcg gatatttctg atgagtcgaa<br />
 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 61&nbsp; aaattatctt gataaagcag gaattactac tgcttgttta cgaattaaat cgaagtggac<br />
@@ Line 121: / Line 111: @@
 &nbsp;&nbsp;&nbsp;&nbsp; 5281 tgggaaaagt tactgtagcc gacgttttgg cggcgcaacc tgtgacgaca aatctgctca<br />
 &nbsp;&nbsp;&nbsp;&nbsp; 5341 aatttatgcg cgcttcgata aaaatgattg gcgtatccaa cctgca<br />
-</font></div>
+</font>

@@ Line 10: / Line 10: @@
 <h2><span class="mw-headline">References</span></h2>
 <ol class="references">
-     <li id="cite_note-0"><strong>^</strong> Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M., &quot;Nucleotide sequence of bacteriophage phi X174 DNA&quot;, <em>Nature</em>. 1977 Feb 24;265(5596):687-95 </li>
+     <li id="cite_note-0"><strong>^</strong> Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M., &quot;Nucleotide sequence of bacteriophage phi X174 DNA&quot;, <em>Nature</em>. <strong>1977</strong> Feb 24;265(5596):687-95 </li>
-     <li id="cite_note-1"><strong>^</strong> Fiers, W., and R. L. Sinsheimer, &quot;The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure&quot;, <em>J. Mol. Biol.</em> <strong>5:</strong>424-434, 1962 </li>
+     <li id="cite_note-1"><strong>^</strong> Fiers, W., and R. L. Sinsheimer, &quot;The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure&quot;, <em>J. Mol. Biol.</em> <strong>5:</strong>424-434, <strong>1962</strong> </li>
-     <li id="cite_note-2"><strong>^</strong> <cite style="FONT-STYLE: normal">Smith, Hamilton O.; Clyde A. Hutchison, Cynthia Pfannkoch, J. Craig Venter (2003-12-23). &quot;Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides&quot;. <em>Proceedings of the National Academy of Sciences</em> <strong>100</strong> (26): 15440-15445. doi:<span class="neverexpand">10.1073/pnas.2237126100</span><span class="reference-accessdate">. Retrieved on 2007-10-08.</span></cite><span class="reference-accessdate"><span class="Z3988" title="ctx_ver=Z39.88-2004&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&amp;rft.genre=article&amp;rft.atitle=Generating+a+synthetic+genome+by+whole+genome+assembly%3A+%7Bphi%7DX174+bacteriophage+from+synthetic+oligonucleotides&amp;rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences&amp;rft.date=2003-12-23&amp;rft.volume=100&amp;rft.issue=26&amp;rft.aulast=Smith&amp;rft.aufirst=Hamilton+O.&amp;rft.pages=15440-15445&amp;rft_id=info:doi/10.1073%2Fpnas.2237126100&amp;rft_id=http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fcontent%2Fabstract%2F100%2F26%2F15440"><span style="DISPLAY: none">&nbsp;</span></span></span> </li>
+     <li id="cite_note-2"><strong>^</strong> <cite style="FONT-STYLE: normal">Smith, Hamilton O.; Clyde A. Hutchison, Cynthia Pfannkoch, J. Craig Venter (2003-12-23). &quot;Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides&quot;. <em>Proceedings of the National Academy of Sciences</em> <strong>100</strong> (26): 15440-15445. doi:<span class="neverexpand">10.1073/pnas.2237126100</span><span class="reference-accessdate">. Retrieved on <strong>2007</strong>-10-08.</span></cite><span class="reference-accessdate"><span class="Z3988" title="ctx_ver=Z39.88-2004&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&amp;rft.genre=article&amp;rft.atitle=Generating+a+synthetic+genome+by+whole+genome+assembly%3A+%7Bphi%7DX174+bacteriophage+from+synthetic+oligonucleotides&amp;rft.jtitle=Proceedings+of+the+National+Academy+of+Sciences&amp;rft.date=2003-12-23&amp;rft.volume=100&amp;rft.issue=26&amp;rft.aulast=Smith&amp;rft.aufirst=Hamilton+O.&amp;rft.pages=15440-15445&amp;rft_id=info:doi/10.1073%2Fpnas.2237126100&amp;rft_id=http%3A%2F%2Fwww.pnas.org%2Fcgi%2Fcontent%2Fabstract%2F100%2F26%2F15440"><span style="DISPLAY: none">&nbsp;</span></span></span> </li>
 </ol>
 <p>&nbsp;</p>

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 <h2><span class="mw-headline">External links</span></h2>
 <ul>
-     <li><a class="external text" title="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" href="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" rel="nofollow">Description &amp; Restriction Map: PhiX174 DNA</a> </li>
+     <li><a class="external text" title="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm" rel="nofollow" href="http://www.fermentas.com/techinfo/nucleicacids/mapfx174.htm">Description &amp; Restriction Map: PhiX174 DNA</a> </li>
 </ul>
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