http://Opengenome.net/index.php?action=history&feed=atom&title=Oil_RedO_staining 2026-05-12T12:10:50Z Revision history for this page on the wiki MediaWiki 1.31.3 http://Opengenome.net/index.php?title=Oil_RedO_staining&diff=25954&oldid=prev 2006-11-22T12:39:51Z

<p></p> <p><b>New page</b></p><div>&lt;font size=&quot;2&quot;&gt;&lt;strong&gt;&lt;font style=&quot;BACKGROUND-COLOR: #ccffcc&quot; size=&quot;4&quot;&gt;Oil RedO staining protocol&lt;/font&gt;&lt;/strong&gt;&lt;br /&gt;<br /> &lt;br /&gt;<br /> &lt;br /&gt;<br /> 1. Freezing slide를 60도씨 dry oven에서 30분 정도 말린다.&lt;br /&gt;<br /> &lt;br /&gt;<br /> 2. Distilled water로 1~2분간 washing한다.&lt;br /&gt;<br /> &lt;br /&gt;<br /> 3. Absolute propylene glycol을 2분간 처리한다.&lt;br /&gt;<br /> &lt;br /&gt;<br /> 4. Oil redO solution에 16시간(over-night) 처리한다.&lt;br /&gt;<br /> &amp;nbsp;* en face ; 2시간 정도&lt;br /&gt;<br /> &amp;nbsp;* aortic sinus ; over-night&lt;br /&gt;<br /> &lt;br /&gt;<br /> 5. 85% propylene glycol 을 1분간 처리한다.&lt;br /&gt;<br /> &lt;br /&gt;<br /> 6. Distilled water로 2~3번, 1분씩 washing한다.&lt;br /&gt;<br /> &lt;br /&gt;<br /> 7. Crystal mount로 mounting. Cover glass 덮을 때는 기포가 발생하지 않도록 유의한다.&lt;br /&gt;<br /> &lt;br /&gt;<br /> &lt;strong&gt;&lt;font color=&quot;#ff6600&quot;&gt;&lt;br /&gt;<br /> ** 결과 : Lipid, 병변이 빨갛게 염색되는 것을 현미경을 통해 확인할 수 있다. **&lt;/font&gt;&lt;/strong&gt;&lt;/font&gt;</div>

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