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<p>&nbsp;</p> <p style="text-align: center">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span style="background-color: #ffffff">&nbsp;&nbsp;</span><span style="color: #0000ff"><span style="background-color: #ffffff"><span style="font-size: xx-large"><b><span style="font-family: Times New Roman">K</span></b></span></span></span><span style="color: #000000"><span style="font-size: xx-large"><b><span style="font-family: Times New Roman">orean</span></b></span></span><span style="font-size: xx-large"><span style="color: #808080"><b><span style="font-family: Times New Roman"> <span style="color: #cc0000">P</span><span style="color: #333333"><span style="background-color: #ffffff">ersonal</span></span><span style="background-color: #ffffff"> </span><span style="color: #cc0000"><span style="background-color: #ffffff">G</span></span><span style="color: #666666">enome</span> <span style="color: #cc0000">P</span></span></b></span></span><span style="color: #999999"><span style="font-size: xx-large"><b><span style="font-family: Times New Roman">roject</span></b></span></span><span style="color: #c0c0c0"><b><span style="font-size: large"><span style="font-family: Comic Sans MS">&nbsp;</span></span></b></span><span style="color: #808080"><span style="font-size: large"><span style="font-family: Comic Sans MS">&nbsp;&nbsp;<img style="width: 126px; height: 125px" alt="" width="130" height="130" src="/userfiles/1.png" /></span></span>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p> <h2>Introduction</h2> <div>Korean Personal Genome Project (KPGP) is a participative research project established by <a href="http://genomefoundation.org/index.php/Main_Page">Genome Research Foundation</a> (GRF). The international <a href="http://www.personalgenomes.org/">Personal Genome Project</a> (PGP), led by U.S. none-profit research group, is founded in 2006 and seeking a diverse range of volunteers with the purpose of improving human health. In concordance with the international PGP, KPGP has started to improve Korean people's health and medical welfare.</div> <h2><br /> Vision</h2> <div>KPGP is aiming to advance science research, in order to promote human health and welfare using genome technology.</div> <h2>Mission</h2> <ul> <li>&nbsp;to build a standard Korean genetic information database</li> <li>&nbsp;to activate the participative genome research</li> <li>&nbsp;to develop genome sequencing and analysis technologies in the age of personal genetic information services</li> <li>&nbsp;to establish an open community if sharing ethical, social, and legal issued in regard to genetic information</li> </ul> <h2>Research</h2> <ul> <li>Korean reference genome project</li> <li>Rare diseases reference genome project</li> <li>HLA alleles reference standard data production project</li> </ul> <h2>Participation&nbsp;</h2> <p><b>Genetic Information Participation</b></p> <p>It is a public participation. The human genetics education, screening and participant`s willingness determine an individual's participation.&nbsp;</p> <p><b>Research Participation </b></p> <p>People who have research experiences in the field of human genetics, or who are willing to contribute to KPGP can participate through screening.&nbsp;</p> <p><b>Company Participation </b></p> <p>Institutes and companies could participate. Sponsorship can greatly help KPGP`s activation.</p> <p><br /> Human genome researches have developed rapidly since the human genome project was completed in 2003. Recently large-scale production of human genome data is available with the advent of Next Generation Sequencing (NGS).</p> <p>Genome Research Foundation (GRF), as a non-profit foundation, determines to open Korean genome data and the current available data of human genome sequence and analysis for free to allow researchers to study. Under the circumstances, OPEN KPGP started on 2011.</p> <p>&nbsp;</p> <h2>How to download</h2> <p>After accessing to the OPEN KPGP website, select the desired data from DATA DOWNLOAD to download it through the Web and FTP. <br /> Please understand that the downloading may be very slow due to Network load. (The speed of downloading 20 people&rsquo;s data through KT cloud is stable, please use it as an alternative.)</p> <p>&nbsp;</p> <h2>Hard disk request</h2> <p>You can get data hard disks by requesting it to the administrator. However, the hard disk and processing costs will occur. <br /> Cost</p> <ul> <li>Hard disk</li> <li>Operating costs (labor and server equipment operating costs).</li> <li>Shipping : door to door delivery(domestic only) / international shipping : please contact. (<a href="mailto:info@kpgp.kr">info@kpgp.kr</a>)</li> </ul> <p>1) Raw sequencing data</p> <table style="width: 242px; height: 95px" border="1" cellspacing="1" cellpadding="1" width="242"> <tbody> <tr> <td>Sample no.</td> <td>Cost(excluding V.A.T)</td> </tr> <tr> <td>10</td> <td>115,000 (KRW)&nbsp;</td> </tr> <tr> <td>20</td> <td>170,000 (KRW)</td> </tr> <tr> <td>30</td> <td>260,000 (KRW)</td> </tr> <tr> <td>40</td> <td>340,000 (KRW)</td> </tr> </tbody> </table> <p>&nbsp;2) Data included : raw sequencing data, aligned data, genome variation, nonsynonymous SNVs</p> <p> <table style="width: 242px; height: 95px" border="1" cellspacing="1" cellpadding="1" width="242"> <tbody> <tr> <td>Sample no.</td> <td>Cost(excluding V.A.T)</td> </tr> <tr> <td>10</td> <td>310,000 (KRW)&nbsp;</td> </tr> <tr> <td>20</td> <td>530,000 (KRW)&nbsp;</td> </tr> <tr> <td>30</td> <td>750,000 (KRW)</td> </tr> <tr> <td>40</td> <td>880,000 (KRW)</td> </tr> </tbody> </table> </p> <p>&nbsp;</p> <p>&nbsp;</p> <h2><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">Method of Sample (gDNA) Preparation for Sending out</span>&nbsp;</h2> <p><span style="font-size: small"><b><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1.&nbsp;Sample(gDNA) Preparation</span></b></span></p> <p>1)&nbsp;&nbsp;DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p> <p>2)&nbsp;&nbsp;Do Not Dissolve in Water</p> <p>&nbsp;</p> <p><b><span style="font-size: small"><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2.&nbsp;Sample(gDNA) Requirement</span></span></b></p> <p>1)&nbsp;&nbsp;&nbsp;Amount and concentration spec.&nbsp;: over 20 ug of gDNA with &gt;50 ng/ul</p> <p>&nbsp;This amount is required for</p> <p>a.&nbsp;&nbsp;Whole genome sequencing (30X)</p> <p>b.&nbsp;&nbsp;Whole exome sequencing (50X)</p> <p>c.&nbsp;&nbsp;Genotyping</p> <p>2)&nbsp;&nbsp;Sample(gDNA) should be&nbsp;: OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p> <p>&nbsp;</p> <p><b><span style="font-size: small"><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3.&nbsp;Sample(gDNA) Packing Information&nbsp;</span></span></b></p> <p>This way is only available when DNA is dissolved in TE or Tris buffer.</p> <p>1)&nbsp;&nbsp;If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p> <p>2)&nbsp;&nbsp;Mark sample information on tube (concentration, sample name&hellip;what you want)</p> <p>3)&nbsp;&nbsp;Seal the cap of tube with parafilm well</p> <p>4)&nbsp;&nbsp;Wrap the tube with paper towel or equivalent</p> <p>5)&nbsp;&nbsp;Put into the 50 ml Falcon tube and close the cap</p> <p>6)&nbsp;&nbsp;Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p> <p>&nbsp;</p> <p><b><span style="font-size: small"><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4.&nbsp;Requirement of Sample(gDNA) Information</span></span></b></p> <p>1)&nbsp; Sample type: DNA</p> <p>2)&nbsp; Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p> <p>3)&nbsp; Gel image of DNA electrophoresis</p> <p>4)&nbsp; Species</p> <p>5)&nbsp; No. of Tubes</p> <p>6)&nbsp; Concentration (ng/&mu;l)</p> <p>7)&nbsp; Volume (&mu;l)</p> <p>8) &nbsp;Total Quantity (&mu;g)</p> <p>9)&nbsp; OD260/280</p> <p>10)&nbsp; OD260/230</p> <p>&nbsp;</p> <p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p> <p>1.<span style="color: #800000"><a href="/userfiles/Method of Sample(gDNA) Preparation for Sending out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p> <p>2.<span style="color: #800000"><a href="/userfiles/Declaration_of_Shipment_for_anyone(2).doc">Declaration of Shipment</a><br /> &nbsp;</span></p> <p>&nbsp;</p> <h2>How it works</h2> <p>&nbsp;</p> <p><img alt="" width="840" height="728" src="/userfiles/chart1(1).gif" /></p> <p>&nbsp;</p> <p>&nbsp;</p> <p><br /> &nbsp;&nbsp;&nbsp;&nbsp; <br /> Data Usage <br /> 1. The genome data&nbsp;is provided by Genome Research Foundation. Anyone can use the data freely.</p> <p>2. People who would like to acquire further information about the data for research publication and collaboration, please contact us. <br /> Contact: <a href="mailto:info@kpgp.kr">info@kpgp.kr</a> / 82-31-888-9327<br /> &nbsp;</p>

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