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<p><strong>Nuclear magnetic resonance</strong> (<strong>NMR</strong>) is a physical phenomenon based upon the quantum mechanical magnetic properties of an atom's nucleus. NMR also commonly refers to a family of scientific methods that exploit nuclear magnetic resonance to study molecules.</p> <p>All nuclei that contain odd numbers of protons or neutrons have an intrinsic magnetic moment and angular momentum. The most commonly measured nuclei are hydrogen-1 (the most receptive isotope at natural abundance) and carbon-13, although nuclei from isotopes of many other elements (e.g. ¹⁵N, ¹⁴N ¹⁹F, ³¹P, ¹⁷O, ²⁹Si, ¹⁰B, ¹¹B, ²³Na, ³⁵Cl, ¹⁹⁵Pt) can also be observed.</p> <p>NMR resonant frequencies for a particular substance are directly proportional to the strength of the applied magnetic field, in accordance with the equation for the Larmor precession frequency.</p> <p>NMR studies magnetic nuclei by aligning them with an applied constant magnetic field and perturbing this alignment using an alternating magnetic field, those fields being orthogonal. The resulting response to the perturbing magnetic field is the phenomenon that is exploited in NMR spectroscopy and magnetic resonance imaging, which use very powerful applied magnetic fields in order to achieve high resolution spectra, details of which are described by the chemical shift and the Zeeman effect.</p> <p>NMR phenomena are also utilised in low field NMR and Earth's field NMR spectrometers, and some kinds of magnetometer.</p> <p>&nbsp;</p> <p><span class="mw-headline"><font size="5">History</font></span></p> <p>&nbsp;</p> <p><span class="mw-headline"><font size="4">Discovery</font></span></p> <p>Nuclear magnetic resonance was first described and measured in molecular beams by Isidor Rabi in 1938.<sup class="reference" id="_ref-0">[1]</sup> Eight years later, in 1946, Felix Bloch and Edward Mills Purcell refined the technique for use on liquids and solids, for which they shared the Nobel Prize in physics in 1952.</p> <p>Purcell had worked on the development and application of RADAR during World War II at Massachusetts Institute of Technology's Radiation Laboratory. His work during that project on the production and detection of radiofrequency energy, and on the absorption of such energy by matter, preceded his discovery of NMR.</p> <p>They noticed that magnetic nuclei, like ¹H and ³¹P, could absorb RF energy when placed in a magnetic field of a strength specific to the identity of the nuclei. When this absorption occurs, the nucleus is described as being <em>in resonance</em>. Interestingly, for analytical scientists, different atoms within a molecule <em>resonate</em> at different frequencies at a given field strength. The observation of the resonance frequencies of a molecule allows a user to discover structural information about the molecule.</p> <p>The development of nuclear magnetic resonance as a technique of analytical chemistry and biochemistry parallels the development of electromagnetic technology and its introduction into civilian use.</p> <p>&nbsp;</p> <p><span class="mw-headline"><font size="5">Theory of nuclear magnetic resonance</font></span></p> <p>&nbsp;</p> <h3><span class="mw-headline">Nuclear spin and magnets</span></h3> <p>The elementary particles, neutrons and protons, composing an atomic nucleus, have the intrinsic quantum mechanical property of spin. The overall spin of the nucleus is determined by the spin quantum number <em>I</em>. If the number of both the protons and neutrons in a given isotope are even then <em>I</em> = 0, i.e. there is no overall spin; just as electrons pair up in atomic orbitals, so do even numbers of protons and neutrons (which are also spin &frac12; particles and hence fermions) pair up giving zero overall spin. In other cases, however, the overall spin is non-zero. For example ²⁷Al has an overall spin <em>I</em> = 5/2.</p> <p>A non-zero spin is associated with a non-zero magnetic moment, &mu;, via</p> <dl><dd><img class="tex" alt="\ \mu = \gamma I " src="http://upload.wikimedia.org/math/9/9/b/99bb4d2a962487f40b66cec92638c6bd.png" /> </dd></dl> <p>where the proportionality constant, &gamma;, is the gyromagnetic ratio. It is this magnetic moment that is exploited in NMR.</p> <p>Electron spin resonance is a related technique which exploits the spin of electrons instead of nuclei. The basic principles are otherwise similar.</p> <p>&nbsp;</p> <h4><span class="mw-headline">Values of spin angular momentum</span></h4> <p>The angular momentum associated with nuclear spin is quantized. This means both that the magnitude of angular momentum is quantized (i.e. <em>I</em> can only take on a restricted range of values), and also that the 'orientation' of the associated angular momentum is quantized. The associated quantum number is known as the magnetic quantum number, <em>m</em>, and can take values from +<em>I</em> to &ndash;<em>I</em> in integral steps. Hence for any given nucleus, there is a total of 2<em>I</em>+1 angular momentum states.</p> <p>The <em>z</em> component of the angular momentum vector, <em>I</em>_<em>z</em>, is therefore:</p> <dl><dd><img class="tex" alt="I_z = m \hbar" src="http://upload.wikimedia.org/math/e/e/f/eefd96b1a43c9a6427b29ca1d6f54426.png" /> </dd></dl> <p>where <img class="tex" alt="\hbar" src="http://upload.wikimedia.org/math/9/d/f/9dfd055ef1683b053f1b5bf9ed6dbbb4.png" /> is Planck's reduced constant.</p> <p>The <em>z</em> component of the magnetic moment is simply</p> <dl><dd><img class="tex" alt="\mu_z = \gamma I_z = m\gamma \hbar" src="http://upload.wikimedia.org/math/1/b/e/1be438efcf67874161d7a25a3b1bbd4b.png" /> </dd></dl> <p>&nbsp;</p> <h4><span class="mw-headline">Spin behavior in a magnetic field</span></h4> <p>Consider nuclei which have a spin of one-half, like ¹H, ¹³C or ¹⁹F. The nucleus has two possible spin states: <em>m</em> = &frac12; or <em>m</em> = -&frac12; (also referred to as up and down or &alpha; and &beta;, respectively). The energies of these states are degenerate&mdash;that is to say that they are the same. Hence the <em>populations</em> of the two states (i.e. number of atoms in the two states) will be approximately equal at thermal equilibrium.</p> <div class="floatright"><span><img height="195" alt="Splitting of nuclei spin states in an external magnetic field" width="300" border="0" src="http://upload.wikimedia.org/wikipedia/commons/thumb/a/a9/EPR_splitting.jpg/300px-EPR_splitting.jpg" /></span></div> <p>If a nucleus is placed in a magnetic field, however, the interaction between the nuclear magnetic moment and the external magnetic field mean the two states no longer have the same energy. The energy of a magnetic moment &mu; when in a magnetic field <em>B</em>₀ (the zero subscript is used to distinguish this magnetic field from any other applied field) is given by the negative scalar product of the vectors:</p> <dl><dd><img class="tex" alt="E = -{\mathbf B_0}\cdot{\mathbf \mu}= - \mu_z B_0" src="http://upload.wikimedia.org/math/c/a/a/caa447d9fefe8dcdeb1ba0804e52d0b8.png" /> </dd></dl> <p>where the magnetic field has been oriented along the <em>z</em> axis.</p> <p>Hence</p> <dl><dd><img class="tex" alt="E = - m\hbar\gamma B_0" src="http://upload.wikimedia.org/math/2/d/6/2d60c09118320cbf35f00dcdc3f7b0eb.png" /> </dd></dl> <p>As a result the different nuclear spin states have different energies in a non-zero magnetic field. In hand-waving terms, we can talk about the two spin states of a spin &frac12; as being <em>aligned</em> either with or against the magnetic field. If &gamma; is positive (true for most isotopes) then <em>m</em> = &frac12; is the lower energy state.</p> <p>The energy difference between the two states is</p> <dl><dd><img class="tex" alt="\Delta E = \hbar\gamma B_0" src="http://upload.wikimedia.org/math/8/f/8/8f87ea3668cfe024980bc63c56ad0b8e.png" /> </dd></dl> <p>and this difference results in a small population bias toward the lower energy state.</p> <p>&nbsp;</p> <h4><span class="mw-headline">Resonance</span></h4> <p>Resonant absorption will occur when electromagnetic radiation of the correct frequency to match this energy difference is applied. The energy of a photon is <em>E</em>&nbsp;= <em>h&nu;</em>, where <em>&nu;</em> is its frequency. Hence absorption will occur when</p> <dl><dd><img class="tex" alt="\nu = \frac{\Delta E}{h}= \frac{\gamma B_0}{2\pi}" src="http://upload.wikimedia.org/math/d/4/5/d4591a1a407be91e67a6bdfb1e790c13.png" /> </dd></dl> <p>These frequencies typically correspond to the radio frequency range of the electromagnetic spectrum.</p> <p>It is this resonant absorption that is detected in NMR.</p> <p>&nbsp;</p> <h4><span class="mw-headline">Nuclear shielding</span></h4> <p>It might appear from the above that all nuclei of the same nuclide (and hence the same &gamma;) would resonate at the same frequency. This is not the case. The most important perturbation of the NMR frequency for applications of NMR is the 'shielding' effect of the surrounding electrons. In general, this electronic shielding reduces the magnetic field <em>at the nucleus</em> (which is what determines the NMR frequency). As a result the energy gap is reduced, and the frequency required to achieve resonance is also reduced. This shift of the NMR frequency due to the chemical environment is called the chemical shift, and it explains why NMR is a direct probe of chemical structure.</p> <p>Unless the local symmetry is particularly high, the shielding effect depends on the orientation of the molecule with respect to the external field. In solid-state NMR, magic angle spinning is required to average out this orientation dependence. This is unnecessary in conventional NMR of molecules in solution since rapid molecular tumbling averages out the anisotropic component of the chemical shift.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Relaxation</span></h3> <dl><dd> <div class="boilerplate seealso"><em>For more details on this topic, see Relaxation (NMR).</em></div> </dd></dl> <p>The process called population relaxation refers to nuclei that return to the thermodynamic state in the magnet. This process is also called <em>T</em>₁ relaxation, where <em>T</em>₁ refers to the mean time for an individual nucleus to return to its equilibrium state. Once the population is relaxed, it can be probed again, since it is in the initial state.</p> <p>The precessing nuclei can also fall out of alignment with each other (returning the net magnetization vector to a nonprecessing field) and stop producing a signal. This is called <em>T</em>₂ relaxation. It is possible to be in this state and not have the population difference required to give a net magnetization vector at its thermodynamic state. Because of this, <em>T</em>₁ is always larger (slower) than <em>T</em>₂. This happens because some of the spins were flipped by the pulse and will remain so until they have undergone population relaxation. In practice, the <em>T</em>₂ time is the life time of the observed NMR signal, the free induction decay. In the NMR spectrum, meaning the Fourier transform of the free induction decay, the <em>T</em>₂ time defines the width of the NMR signal. Thus, a nucleus having a large <em>T</em>₂ time gives rise to a sharp signal, whereas nuclei with shorter <em>T</em>₂ times give rise to more broad signals. The length of <em>T</em>₁ and <em>T</em>₂ is closely related to molecular motion.</p> <p>&nbsp;</p> <p><span class="mw-headline"><font size="5">[[NMR spectroscopy]]</font></span></p> <p>NMR spectroscopy is one of the principal techniques used to obtain physical, chemical, electronic and structural information about molecules due to the chemical shift and Zeeman effect on the resonant frequencies of the nuclei. It is a powerful technique that can provide detailed information on the topology, dynamics and three-dimensional structure of molecules in solution and the solid state. Also, nuclear magnetic resonance is one of the techniques that has been used to build elementary quantum computers.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Continuous wave (CW) spectroscopy</span></h3> <p>In its first few decades, nuclear magnetic resonance spectrometers used a technique known as continuous-wave (CW) spectroscopy. Although NMR spectra could be obtained using a fixed magnetic field and sweeping the frequency of the electromagnetic radiation, this more typically involved using a fixed frequency source and varying the current (and hence magnetic field) in an electromagnet to observe the resonant absorption signals. (This is the origin of the now anachronistic but still common &quot;high&quot; and &quot;low&quot; field terminology for low frequency and high frequency regions respectively of the NMR spectrum.)</p> <p>CW spectroscopy is inefficient in comparison to Fourier techniques (see below) as it probes the NMR response at individual frequencies in succession. As the NMR signal is intrinsically weak, the observed spectra suffer from a poor signal-to-noise ratio (S/N). This can be mitigated by signal averaging i.e. adding the spectra from repeated measurements. While the NMR signal is constant between scans and so adds linearly, the random noise adds more slowly &mdash; as the square-root of the number of spectra (see Random walk). Hence the overall ratio of the signal to the noise increases as the square-root of the number of spectra measured.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Fourier spectroscopy</span></h3> <p>Most applications of NMR involve full NMR spectra, that is, the intensity of the NMR signal as a function of frequency. Early attempts to acquire the NMR spectrum more efficiently than simple CW methods involved irradiating simultaneously with more than one frequency. It was soon realised, however, that a simpler solution was to use short pulses of radio-frequency (centred at the middle of the NMR spectrum). In simple terms, a short square pulse of a given &quot;carrier&quot; frequency &quot;contains&quot; a range of frequencies centred about the carrier frequency, with the range of excitation (bandwidth) being inversely proportional to the pulse duration (the Fourier transform of an approximate square wave contains contributions from all the frequencies in the neighborhood of the principal frequency). The restricted range of the NMR frequencies made it relatively easy to use RF pulses to excite the entire NMR spectrum.</p> <p>Applying such a pulse to a set of nuclear spins simultaneously excites all the NMR transitions. In terms of the net magnetisation vector, this corresponds to tilting the magnetisation vector away from its equilibrium position (aligned along the external magnetic field). The out-of-equilibrium magnetisation vector precesses about the external magnetic field at the NMR frequency of the spins. This oscillating magnetisation induces a current in a nearby pickup coil, creating an electrical signal oscillating at the NMR frequency. This signal is known as the free induction decay (FID) and contains the sum of the NMR responses from all the excited spins. In order to obtain the frequency-domain NMR spectrum (intensity vs. frequency) this time-domain signal (intensity vs. time) must be Fourier transformed. Fortunately the development of FT-NMR coincided with the development of digital computers and Fast Fourier Transform algorithms.</p> <p>Richard R. Ernst was one of the pioneers of pulse (FT) NMR and won a Nobel Prize in chemistry in 1991 for his work on FT-NMR and his development of multi-dimensional NMR (see below).</p> <p>&nbsp;</p> <h3><span class="mw-headline">Multi-dimensional</span></h3> <p>The use of pulses of different shapes, frequencies and durations in specifically-designed patterns or <em>pulse sequences</em> allows the spectroscopist to extract many different types of information about the molecule.</p> <p>Multi-dimensional nuclear magnetic resonance spectroscopy is a kind of FT-NMR in which there are at least two pulses and, as the experiment is repeated, the pulse sequence is varied. In <em>multidimensional nuclear magnetic resonance</em> there will be a sequence of pulses and, at least, one variable time period. In three dimensions, two time sequences will be varied. In four dimensions, three will be varied.</p> <p>There are many such experiments. In one, these time intervals allow&mdash;among other things&mdash;magnetization transfer between nuclei and, therefore, the detection of the kinds of nuclear-nuclear interactions that allowed for the magnetization transfer. Interactions that can be detected are usually classified into two kinds. There are <em>through-bond</em> interactions and <em>through-space</em> interactions, the latter usually being a consequence of the nuclear Overhauser effect. Experiments of the nuclear-Overhauser variety may establish distances between atoms.</p> <p>Although the fundamental concept of 2D NMR was proposed by the Belgian scientist Jean Jeener, professor at the Free University of Brussels (now split into the Universit&eacute; Libre de Bruxelles and the Vrije Universiteit Brussel), this idea was largely developed by Richard Ernst who won the 1991 Nobel prize in Chemistry for his work in FT and multi-dimensional NMR. Multi-dimensional NMR experiments were further developed into powerful methodologies for studying biomolecules in solution, in particular for the determination of the structure of biopolymers such as proteins or even small nucleic acids. Kurt W&uuml;thrich shared the 2002 Nobel Prize in Chemistry for his work in protein nuclear magnetic resonance spectroscopy.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Solids</span></h3> <p>This technique complements biopolymer X-ray crystallography in that it is frequently applicable to biomolecules in a liquid or liquid crystal phase, whereas crystallography, as the name implies, is performed on molecules in a solid phase. Though nuclear magnetic resonance is used to study solids, extensive atomic-level biomolecular structural detail is especially challenging to obtain in the solid state. There is no signal averaging by thermal motion in the solid state, where molecules are held still, each in a slightly different electronic environment, giving a different signal. This variation in electronic environment lowers resolution greatly and makes interpretation more difficult. Raymond Andrew was a pioneer in the development of high-resolution solid-state nuclear magnetic resonance. He introduced the magic angle spinning (MAS) technique and allowed for an increase in resolution by several orders of magnitude. In MAS, the sample is averaged by spinning it at several kilohertz.</p> <p>Alex Pines together with John S. Waugh revolutionized the area with the introduction of the cross-polarization technique in order to enhance low abundance and sensitivity nuclei.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Sensitivity</span></h3> <p>Because the intensity of nuclear magnetic resonance signals and, hence, the sensitivity of the technique depends on the strength of the magnetic field the technique has also advanced over the decades with the development of more powerful magnets. Advances made in audio-visual technology have also improved the signal-generation and processing capabilities of newer machines.</p> <p>The sensitivity of nuclear magnetic resonance signals is also dependent&mdash;as noted above&mdash;on the presence of a magnetically-susceptible nuclide and, therefore, either on the natural abundance of such nuclides or on the ability of the experimentalist to artificially enrich the molecules, under study, with such nuclides. The most abundant naturally-occurring isotopes of hydrogen and phosphorus&mdash;for instance&mdash;are both magnetically susceptible and readily useful for nuclear magnetic resonance spectroscopy. In contrast, carbon and nitrogen have useful isotopes but which occur only in very low natural abundance.</p> <p>Other limitations on sensitivity arise from the quantum-mechanical nature of the phenomenon. For quantum states separated by energy equivalent to radio frequencies, thermal energy from the environment causes the populations of the states to be close to equal. Since incoming radiation is equally likely to cause stimulated emission (a transition from the upper to the lower state) as absorption, the NMR effect depends on an excess of nuclei in the lower states. Several factors can reduce sensitivity, including</p> <ul> <li>Increasing temperature, which evens out the population of states. Conversely, low temperature NMR can sometimes yield better results than room-temperature NMR, providing the sample remains liquid. </li> <li>Saturation of the sample with radio energy. This manifests itself in both CW and pulsed NMR, the first by using too much continuous power, and the second by pulsing frequently without allowing time for the nuclei to return to thermal equilibrium. For nuclei such as ²⁹Si this is a serious problem as the relaxation time is measured in seconds. </li> <li>Non-magnetic effects, such as electric-quadrupole coupling of spin-1 and spin-3/2 nuclei with their local environment, which broaden and weaken absorption peaks. ¹⁴N, a very common spin-1 nucleus, is difficult to study for this reason. High resolution NMR instead probes molecules using the rarer Nitrogen ¹⁵N isotope, which has spin-&frac12;. </li> </ul> <p>&nbsp;</p> <h3><span class="mw-headline">Isotopes</span></h3> <p>Many chemical elements can be used for NMR analysis. [1]</p> <ul> <li><strong>¹H</strong>, the most commonly used, very useful. Highly abundant, the most sensitive nucleus apart from tritium. Narrow chemical shift, but sharp signals. In particular, the ¹H signal is that used in magnetic resonance imaging. </li> <li><strong>²H</strong>, commonly used in the form of deuterated solvents to avoid interference of solvents in measurement of ¹H. Rarely used in NMR measurents themselves, due to low resolution and low sensitivity; mainly utilized in determining of effectiveness of chemical deuteration. </li> <li><strong>³He</strong>, very sensitive. Low percentage in natural helium, has to be enriched. Used mainly in studies of endohedral fullerenes. </li> <li><strong>¹³C</strong>, commonly used. Low percentage in natural carbon, therefore spectrum acquisition takes a long time. Frequently used for labeling of compounds in synthetic and metabolic studies. Has low sensitivity and wide chemical shift, yields sharp signals. Low percentage makes it useful by preventing spin-spin couplings and makes the spectrum appear less crowded. </li> <li><strong>¹⁵N</strong>, relatively commonly used. Can be used for labeling compounds. Nucleus very insensitive but yields sharp signals. Low percentage in natural nitrogen together with low sensitivity requires high concentrations or expensive isotope enrichment. </li> <li><strong>¹⁴N</strong>, medium sensitivity nucleus with wide chemical shift. Its large quadrupole moment interferes in acquisition of high resolution spectra, limiting usefulness to smaller molecules. </li> <li><strong>¹⁹F</strong>, relatively commonly measured. Sensitive, yields sharp signals, has wide chemical shift. </li> <li><strong>³¹P</strong>, 100% of natural phosphorus. Medium sensitivity, wide chemical shift range, yields sharp lines. Used in biochemical studies. </li> <li><strong>¹⁷O</strong>, low sensitivity and very low natural abundance. </li> <li><strong>¹⁰B</strong>, lower sensitivity than ¹¹B. Use quartz tubes, as borosilicate glass interferes with measurement. </li> <li><strong>¹¹B</strong>, more sensitive than ¹⁰B, yields sharper signals. Use quartz tubes, as borosilicate glass interferes with measurement. </li> <li><strong>³⁵Cl</strong> and <strong>³⁷Cl</strong>, broad signal. ³⁵Cl significantly more sensitive, preferred over ³⁷Cl despite its slightly broader signal. Organic chlorides yield very broad signals, use limited to inorganic and ionic chlorides and very small organic molecules. </li> <li><strong>⁴³Ca</strong>, used in biochemistry to study calcium binding to DNA, proteins, etc. Moderately sensitive, very low natural abundance, has to be enriched. </li> <li><strong>¹⁹⁵Pt</strong>, used in studies of catalysts and complexes. </li> <li>Other nuclei, usually used in the studies of their complexes and chemical binding, or to detect presence of the element: ⁶Li, ⁷Li, ⁹Be, ²¹Ne, ²³Na, ²⁵Mg, ²⁷Al, ²⁹Si, ³³S, ³⁹K, ⁴⁰K, ⁴¹K, ⁴⁵Sc, ⁴⁷Ti, ⁴⁹Ti, ⁵⁰V, ⁵¹V, ⁵³Cr, ⁵⁵Mn, ⁵⁷Fe, ⁵⁹Co, ⁶¹Ni, ⁶³Cu, ⁶⁵Cu, ⁶⁷Zn, ⁶⁹Ga, ⁷¹Ga, ⁷³Ge, ⁷⁷Se, ⁸¹Br, ⁸⁷Rb, ⁸⁷Sr, ⁹⁵Mo, ¹⁰⁹Ag, ¹¹³Cd, ¹²⁵Te, ¹²⁷I, ¹³³Cs, ¹³⁵Ba, ¹³⁷Ba, ¹³⁹La, ¹⁸³W, ¹⁹⁹Hg. </li> </ul> <p>&nbsp;</p> <p><span class="mw-headline"><font size="5">Applications</font></span></p> <p>&nbsp;</p> <h3><span class="mw-headline">Medicine</span></h3> <p>The use of nuclear magnetic resonance best known to the general public is in magnetic resonance imaging for medical diagnosis, however, it is also widely used in chemical studies, notably in NMR spectroscopy such as proton NMR and carbon-13 NMR. Biochemical information can also be obtained from living tissue (e.g human brain tumours) with the technique known as in vivo magnetic resonance spectroscopy.</p> <p>These studies are possible because nuclei are surrounded by orbiting electrons, which are also spinning charged particles such as magnets and, so, will partially shield the nuclei. The amount of shielding depends on the exact local environment. For example, a hydrogen bonded to an oxygen will be shielded differently than a hydrogen bonded to a carbon atom. In addition, two hydrogen nuclei can interact via a process known as spin-spin coupling, if they are on the same molecule, which will split the lines of the spectra in a recognisable way.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Chemistry</span></h3> <p>By studying the peaks of nuclear magnetic resonance spectra, skilled chemists can determine the structure of many compounds. It can be a very selective technique, distinguishing among many atoms within a molecule or collection of molecules of the same type but which differ only in terms of their local chemical environment.</p> <p>By studying <em>T</em>₂* information a chemist can determine the identity of a compound by comparing the observed nuclear precession frequencies to known frequencies. Further structural data can be elucidated by observing <em>spin-spin coupling</em>, a process by which the precession frequency of a nucleus can be influenced by the magnetization transfer from nearby nuclei. Spin-spin coupling is most commonly observed in NMR involving common isotopes, such as Hydrogen-1 (HNMR).</p> <p><em>T</em>₂ information can give information about dynamics and molecular motion.</p> <p>Because the nuclear magnetic resonance <em>timescale</em> is rather slow, compared to other spectroscopic methods, changing the temperature of a <em>T</em>₂* experiment can also give information about fast reactions, such as the Cope rearrangement or about structural dynamics, such as ring-flipping in cyclohexane.</p> <p>An example of nuclear magnetic resonance being used in the determination of a structure is that of buckminsterfullerene. This now famous form of carbon has 60 carbon atoms forming a sphere. The carbon atoms are all in identical environments and so should see the same internal H field. Unfortunately, buckminsterfullerene contains no hydrogen and so ¹³C nuclear magnetic resonance has to be used. ¹³C spectra require longer acquisition times since carbon-13 is not the common isotope of carbon (unlike hydrogen, where ¹H is the common isotope). However, in 1990 the spectrum was obtained by R. Taylor and co-workers at the University of Sussex and was found to contain a single peak, confirming the unusual structure of C₆₀.<sup class="reference" id="_ref-1">[2]</sup></p> <p>&nbsp;</p> <h3><span class="mw-headline">Non-destructive testing</span></h3> <p>Nuclear magnetic resonance is extremely useful for analyzing samples non-destructively. Radio waves and static magnetic fields easily penetrate many types of matter and anything that is not inherently ferromagnetic. For example, various expensive biological samples, such as nucleic acids, including RNA and DNA, or proteins, can be studied using nuclear magnetic resonance for weeks or months before using destructive biochemical experiments. This also makes nuclear magnetic resonance a good choice for analyzing dangerous samples.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Data acquisition in the petroleum industry</span></h3> <p>Another use for nuclear magnetic resonance is data acquisition in the petroleum industry for petroleum and natural gas exploration and recovery. A borehole is drilled into rock and sedimentary strata into which nuclear magnetic resonance logging equipment is lowered. Nuclear magnetic resonance analysis of these boreholes is used to measure rock porosity, estimate permeability from pore size distribution and identify pore fluids (water, oil and gas). These instruments are typically low field NMR spectrometers.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Process control</span></h3> <p>NMR has now entered the arena of real-time process control and process optimization in oil refineries and petrochemical plants. Two different types of NMR analysis are utilized to provide real time analysis of feeds and products in order to control and optimize unit operations. Time-domain NMR (TD-NMR) spectrometers operating at low field (2-20 MHz for ¹H) yield free induction decay data that can be used to determine absolute hydrogen content values, rheological information, and component composition. These spectrometers are used in mining, polymer production, cosmetics and food manufacturing as well as coal analysis. High resolution FT-NMR spectrometers operating in the 60 MHz range with shielded permanent magnet systems yield high resolution ¹H NMR spectra of refinery and petrochemical streams. The variation observed in these spectra with changing physical and chemical properties is modelled utilizing chemometrics to yield predictions on unknown samples. The prediction results are provided to control systems via analogue or digital outputs from the spectrometer.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Earth's field NMR</span></h3> <p>In the Earth's magnetic field, NMR frequencies are in the audio frequency range. EFNMR is typically stimulated by applying a relatively strong dc magnetic field pulse to the sample and, following the pulse, analysing the resulting low frequency alternating magnetic field that occurs in the earth's magnetic field due to free induction decay (FID). These effects are exploited in some types of magnetometers, EFNMR spectrometers, and MRI imagers[[2]]. Their inexpensive portable nature makes these instruments valuable for field use and for teaching.</p> <p>&nbsp;</p> <h3><span class="mw-headline">Magnetometers</span></h3> <p>Various magnetometers use NMR effects to measure magnetic fields, including proton magnetometers or proton precession magnetometers (PPM), and Overhauser magnetometers. See also Earth's field NMR.</p> <p>&nbsp;</p> <p><span class="mw-headline"><font size="5">Makers of NMR equipment</font></span></p> <p>Major NMR instrument makers include Bruker, General Electric, JEOL, Kimble Chase, Philips, Siemens AG, Varian, Inc. and SpinCore Technologies, Inc.</p> <p>&nbsp;</p> <p><span class="mw-headline"><font size="5">See also</font></span></p> <div> <ul> <li>Carbon-13 NMR </li> <li>Chemical shift </li> <li>Earth's field NMR (EFNMR) </li> <li>Electromagnetism </li> <li>Electron spin/paramagnetic resonance </li> <li>Ferromagnetic resonance </li> <li>Free induction decay (FID) </li> <li>Gyromagnetic ratio </li> <li>In vivo magnetic resonance spectroscopy (MRS) </li> <li>J-coupling </li> <li>Larmor equation (Not to be confused with Larmor formula). </li> <li>Larmor precession </li> <li>Low field NMR </li> <li>Magic angle spinning </li> <li>Magnetic resonance imaging (MRI) </li> <li>Magnetometer </li> <li>Molecular modelling </li> <li>NMR spectroscopy </li> <li>Nuclear quadrupole resonance (NQR) </li> <li>Protein NMR </li> <li>Proton NMR </li> <li>Rabi cycle </li> <li>Relaxometry </li> <li>Relaxation (NMR) </li> <li>Solid-state NMR </li> <li>Zeeman effect </li> <li>&nbsp; </li> <li>&nbsp; </li> <li>&nbsp; </li> </ul> </div> <p><span class="mw-headline"><font size="5">References</font></span></p> <ol class="references"> <li id="_note-0"><strong><a title="" href="http://en.wikipedia.org/wiki/NMR#_ref-0">^</a></strong> Rabi, I. I., Zacharias, J. R., Millman, S. and Kusch, P. (1938). A New Method of Measuring Nuclear Magnetic Moment. Physical Review 53, 318-318 </li> <li id="_note-1"><strong><a title="" href="http://en.wikipedia.org/wiki/NMR#_ref-1">^</a></strong> <span class="Z3988" title="ctx_ver=Z39.88-2004&amp;rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook&amp;rft.genre=book&amp;rft.title=Isolation%2C+separation+and+characterization+of+the+fullerenes+C60+and+C70%3A++the+third+form+of+carbon&amp;rft.aulast=Taylor&amp;rft.aufirst=Roger&amp;rft.date=1990&amp;rft.volume=20&amp;rft.pages=1423-5"><cite id="CITEREFTaylorHare.2C_Jonathan_P..3B_Abdul-Sada.2C_Ala.27a_K..3B_Kroto.2C_Harold_W">Taylor, Roger &amp; Hare, Jonathan P.; Abdul-Sada, Ala'a K.; Kroto, Harold W (1990), &quot;Isolation, separation and characterization of the fullerenes C60 and C70: the third form of carbon&quot;, <em>Journal of the Chemical Society, Chemical Communications</em> <strong>20</strong>: 1423-5</cite></span> </li> </ol> <ol> <li>Martin, G.E; Zekter, A.S., &lsquo;&rsquo;Two-Dimensional NMR Methods for Establishing Molecular Connectivity&rsquo;&rsquo;; VCH Publishers, Inc: New York, 1988 (p.59) </li> <li>Akitt, J.W.; Mann, B.E., &lsquo;&rsquo;NMR and Chemistry&rsquo;&rsquo;; Stanley Thornes: Cheltenham, UK, 2000. (p273) </li> <li>Akitt, J.W.; Mann, B.E., &lsquo;&rsquo;NMR and Chemistry&rsquo;&rsquo;; Stanley Thornes: Cheltenham, UK, 2000. (p287) </li> <li>Hornak, Joseph P. <a class="external text" title="http://www.cis.rit.edu/htbooks/nmr/" rel="nofollow" href="http://www.cis.rit.edu/htbooks/nmr/"><em>The Basics of NMR</em></a> </li> <li><a class="external text" title="http://www.spectroscopynow.com/Spy/basehtml/SpyH/1,,5-14-9-0-0-education_dets-0-1839,00.html" rel="nofollow" href="http://www.spectroscopynow.com/Spy/basehtml/SpyH/1,,5-14-9-0-0-education_dets-0-1839,00.html">J. Keeler, Understanding NMR Spectroscopy</a> </li> <li>Wuthrich, Kurt <em>NMR of Proteins and Nucleic Acids</em> Wiley-Interscience, New York, NY USA 1986. </li> <li>Tyszka, J. M. &amp; Fraser, S. E. &amp; Jacobs, R. E. (2005). <a class="external text" title="http://dx.doi.org/10.1016/j.copbio.2004.11.004" rel="nofollow" href="http://dx.doi.org/10.1016/j.copbio.2004.11.004">Magnetic resonance microscopy: recent advances and applications</a>. <em><a class="mw-redirect" title="Current Opinion in Biotechnology" href="http://en.wikipedia.org/wiki/Current_Opinion_in_Biotechnology">Current Opinion in Biotechnology</a>,</em> <strong>16</strong>(1):93-99. </li> </ol> <p><a id="External_links" name="External_links"></a></p> <p><span class="mw-headline"><font size="5">External links</font></span></p> <ul> <li><a class="external text" title="http://www.vega.org.uk/video/programme/21" rel="nofollow" href="http://www.vega.org.uk/video/programme/21">Richard Ernst, NL - Developer of Multdimensional NMR techniques</a> Freeview video provided by the Vega Science Trust. </li> <li><a class="external text" title="http://www.vega.org.uk/video/programme/115" rel="nofollow" href="http://www.vega.org.uk/video/programme/115">'An Interview with Kurt Wuthrich'</a> Freeview video by the Vega Science Trust (W&uuml;thrich was awarded a Nobel Prize in Chemistry in 2002 &quot;for his development of nuclear magnetic resonance spectroscopy for determining the three-dimensional structure of biological macromolecules in solution&quot;). </li> <li><em><a class="external text" title="http://abbreviations.oxid.ro/Academic-and-Science/Nuclear-Magnetic-Resonance/" rel="nofollow" href="http://abbreviations.oxid.ro/Academic-and-Science/Nuclear-Magnetic-Resonance/">Acronyms, Abbreviations and Initialisms - Nuclear Magnetic Resonance</a></em> </li> <li><a class="external text" title="http://vam.anest.ufl.edu/forensic/nmr.html" rel="nofollow" href="http://vam.anest.ufl.edu/forensic/nmr.html">A free interactive simulation of NMR principles</a> </li> <li><a class="external text" title="http://www.bionmr.com" rel="nofollow" href="http://www.bionmr.com/">Bionmr.com - discussion of NMR applications in biological systems</a> </li> <li><a class="external text" title="http://www.nmrwiki.org" rel="nofollow" href="http://www.nmrwiki.org/"><em>NMRW</em>iki.ORG</a> NMR resource you can edit </li> <li><a class="external text" title="http://www.ismar.org" rel="nofollow" href="http://www.ismar.org/">The International Society of Magnetic Resonance</a> </li> <li><a class="external text" title="http://www.auremol.de" rel="nofollow" href="http://www.auremol.de/">AUREMOL (Universitaet Regensburg)</a> </li> <li>NMR processing software from <a class="external text" title="http://www.acdlabs.com/" rel="nofollow" href="http://www.acdlabs.com/">ACD/Labs</a> for <a class="external text" title="http://www.acdlabs.com/products/spec_lab/exp_spectra/nmr/" rel="nofollow" href="http://www.acdlabs.com/products/spec_lab/exp_spectra/nmr/">1D</a> and <a class="external text" title="http://www.acdlabs.com/products/spec_lab/exp_spectra/2d_nmr/" rel="nofollow" href="http://www.acdlabs.com/products/spec_lab/exp_spectra/2d_nmr/">2D</a> NMR spectra. DB interface available. </li> <li>NMR Prediction software <a class="external text" title="http://www.acdlabs.com/products/spec_lab/predict_nmr/" rel="nofollow" href="http://www.acdlabs.com/products/spec_lab/predict_nmr/">ACD/NMR Predictors</a> </li> <li>Automated elucidation of chemical structures <a class="external text" title="http://www.acdlabs.com/products/spec_lab/complex_tasks/str_elucidator/" rel="nofollow" href="http://www.acdlabs.com/products/spec_lab/complex_tasks/str_elucidator/">ACD/Structure Elucidator</a> </li> <li>NMR simulation software <a class="external text" title="http://www.bpc.lu.se/QSim" rel="nofollow" href="http://www.bpc.lu.se/QSim">QSim</a> </li> <li>NMR processing software <a class="external text" title="http://spin.niddk.nih.gov/bax/software/NMRPipe" rel="nofollow" href="http://spin.niddk.nih.gov/bax/software/NMRPipe">NMRPipe</a> </li> <li><a class="external text" title="http://www.bmrb.wisc.edu/" rel="nofollow" href="http://www.bmrb.wisc.edu/">BMRB</a> BioMagResBank - A repository for experimental data from NMR spectroscopy of proteins, peptides, nucleic acids and small biomolecules. </li> <li><a class="external text" title="http://www.chemistry.ohio-state.edu/~grandinetti/RMN" rel="nofollow" href="http://www.chemistry.ohio-state.edu/~grandinetti/RMN">RMN</a> - An NMR data processing program for the Macintosh. </li> <li><a class="external text" title="http://baker-atlas.bakerhughesdirect.com/NMR-data-acquisition-systems/" rel="nofollow" href="http://baker-atlas.bakerhughesdirect.com/NMR-data-acquisition-systems/">Baker Hughes - NMR data acquisition systems</a> </li> <li><a class="external text" title="http://baker-atlas.bakerhughesdirect.com/NMR-data-logging/" rel="nofollow" href="http://baker-atlas.bakerhughesdirect.com/NMR-data-logging/">Baker Hughes - The benefits of NMR data logging</a> </li> <li><a class="external text" title="http://www.process-nmr.com/time_domain_process_nmr_spectrom.htm" rel="nofollow" href="http://www.process-nmr.com/time_domain_process_nmr_spectrom.htm">Applications of Time-Domain NMR in Process Control</a> </li> <li><a class="external text" title="http://www.process-nmr.com/process.htm" rel="nofollow" href="http://www.process-nmr.com/process.htm">Applications of High Resolution FT-NMR in Real-Time Process Control</a> </li> <li><a class="external text" title="http://nmr.bham.ac.uk" rel="nofollow" href="http://nmr.bham.ac.uk/">HWB-NMR</a> Henry Wellcome Building for Biomolecular NMR, Birmingham, UK. </li> <li><a class="external text" title="http://www.nmr.ch/" rel="nofollow" href="http://www.nmr.ch/">CARA</a> - Computer Aided Resonance Assignment, freeware, developed at the group of Prof. Kurt W&uuml;thrich </li> <li><a class="external text" title="http://www.ccpn.ac.uk/" rel="nofollow" href="http://www.ccpn.ac.uk/">CCPN</a> - The Collaborative Computing Project for NMR, based at the University of Cambridge, UK </li> <li><a class="external text" title="http://structbio.vanderbilt.edu" rel="nofollow" href="http://structbio.vanderbilt.edu/">Vanderbilt Structural Biology</a> General site for structural biology at Vanderbilt University. NMR, x-ray crystallography, and computational biology all included. </li> <li><a class="external text" title="http://janocchio.sourceforge.net" rel="nofollow" href="http://janocchio.sourceforge.net/">Janocchio</a> Conformation-dependent coupling and NOE prediction for small molecules. </li> <li><a class="external text" title="http://www.teachspin.com/instruments/earths_field_NMR/index.shtml" rel="nofollow" href="http://www.teachspin.com/instruments/earths_field_NMR/index.shtml">Earth's field NMR (EFNMR)</a> </li> <li><a class="external text" title="http://www.chem.queensu.ca/FACILITIES/NMR/nmr/webcourse/precess.htm" rel="nofollow" href="http://www.chem.queensu.ca/FACILITIES/NMR/nmr/webcourse/precess.htm">Animation of NMR spin 1/2 precession</a> </li> <li><a class="external text" title="http://www.ebyte.it/stan/blog.html#08Feb29" rel="nofollow" href="http://www.ebyte.it/stan/blog.html#08Feb29">Article on helium scarcity and potential effects on NMR and MRI communities</a> </li> </ul>

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