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<p style="text-align: center"> <span style="background-color: #ffffff"> </span><span style="color: #0000ff"><span style="background-color: #ffffff"><span style="font-size: xx-large"><b><span style="font-family: Times New Roman">K</span></b></span></span></span><span style="color: #000000"><span style="font-size: xx-large"><b><span style="font-family: Times New Roman">orean</span></b></span></span><span style="font-size: xx-large"><span style="color: #808080"><b><span style="font-family: Times New Roman"> <span style="color: #cc0000">P</span><span style="color: #333333"><span style="background-color: #ffffff">ersonal</span></span><span style="background-color: #ffffff"> </span><span style="color: #cc0000"><span style="background-color: #ffffff">G</span></span><span style="color: #666666">enome</span> <span style="color: #cc0000">P</span></span></b></span></span><span style="color: #999999"><span style="font-size: xx-large"><b><span style="font-family: Times New Roman">roject</span></b></span></span><span style="color: #c0c0c0"><b><span style="font-size: large"><span style="font-family: Comic Sans MS"> </span></span></b></span><span style="color: #808080"><span style="font-size: large"><span style="font-family: Comic Sans MS"> <img style="width: 126px; height: 125px" alt="" width="130" height="130" src="/userfiles/1.png" /></span></span> </span> </p>
<h2>Introduction</h2>
<div><span style="font-size: small">Korean Personal Genome Project (KPGP) is a participative research project established by </span><a href="http://genomefoundation.org/index.php/Main_Page"><span style="font-size: small">Genome Research Foundation</span></a><span style="font-size: small"> (GRF). It is a part of PGP (The international </span><a href="http://www.personalgenomes.org/"><span style="font-size: small">Personal Genome Project</span></a><span style="font-size: small"> (PGP), led by U.S. none-profit research group, is founded in 2006 and seeking a diverse range of volunteers with the purpose of improving human health. In concordance with the international PGP, KPGP has started to improve Korean people's health and medical welfare.</div><h2><br />Vision</h2><divspan>KPGP is aiming to advance science research, in order to promote human health and welfare using genome technology.</div>
<h2>Mission</h2>
<ul>
<li>to function as Korean center for PGP (Harvard Medical School, George Church lab)</li>
<li> to build a standard Korean genetic information database</li>
<li> to activate the participative genome research</li>
<p> </p>
<h2>How to download</h2>
<p><span style="font-size: small">After accessing to the OPEN KPGP website, select the desired data from DATA DOWNLOAD to download it through the Web and FTP. <br />Please understand that the downloading may be very slow due to Network load. (The speed of downloading 20 people’s data through KT cloud is stable, please use it as an alternative.)</span></p>
<p> </p>
<h2>Hard disk request</h2>
<p><span style="font-size: small">You can get data hard disks by requesting it to the administrator. However, the hard disk and processing costs will occur. <br />Cost:</span></p>
<ul>
<li><span style="font-size: small">Hard diskdisks</span></li> <li><span style="font-size: small">Operating costs (labor and server equipment operating costs).</span></li> <li><span style="font-size: small">Shipping : door to door delivery(domestic only) / international shipping : please contact. (</span><a href="mailto:info@kpgp.kr"><span style="font-size: small">info@kpgp.kr</span></a><span style="font-size: small">)</span></li>
</ul>
<p><span style="font-size: small">1) Raw sequencing data</span></p>
<table style="width: 242px; height: 95px" border="1" cellspacing="1" cellpadding="1" width="242">
<tbody>
<h2><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">Method of Sample (gDNA) Preparation for Sending out</span> </h2>
<p><span style="font-size: small"><b><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">1. Sample(gDNA) Preparation</span></b></span></p>
<p><span style="font-size: small">1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</span></p><p><span style="font-size: small">2) Do Not Dissolve in Water</span></p>
<p> </p>
<p><b><span style="font-size: small"><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2. Sample(gDNA) Requirement</span></span></b></p>
<p><span style="font-size: small">1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul</span></p><p><span style="font-size: small"> This amount is required for</span></p><p><span style="font-size: small">a. Whole genome sequencing (30X)</span></p><p><span style="font-size: small">b. Whole exome sequencing (50X)</span></p><p><span style="font-size: small">c. Genotyping</span></p><p><span style="font-size: small">2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</span></p><p><span style="font-size: small"> </span></p>
<p><b><span style="font-size: small"><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3. Sample(gDNA) Packing Information </span></span></b></p>
<p><span style="font-size: small">This way is only available when DNA is dissolved in TE or Tris buffer.</span></p><p><span style="font-size: small">1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service</span></p><p><span style="font-size: small">2) Mark sample information on tube (concentration, sample name…what you want)</span></p><p><span style="font-size: small">3) Seal the cap of tube with parafilm well</span></p><p><span style="font-size: small">4) Wrap the tube with paper towel or equivalent</span></p><p><span style="font-size: small">5) Put into the 50 ml Falcon tube and close the cap</span></p><p><span style="font-size: small">6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent</span></p>
<p> </p>
<p><b><span style="font-size: small"><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4. Requirement of Sample(gDNA) Information</span></span></b></p>
<p><br />
<br />
<span style="font-size: medium">Data Usage </span><br />
1. The genome data is provided by Genome Research Foundation. Anyone can use the data freely.</p>
<p>2. People who would like to acquire further information about the data for research publication and collaboration, please contact us. <br />
Contact: <a href="mailto:info@kpgp.kr">info@kpgp.kr</a> / 82-31-888-9327</p><p> </p><p><br />
</p>
