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<h2h1>Informaion of sample(gDNA) delivery preparation</h2><p>1) Method of gDNA delivery preparation</ph1><ul> <lih1> DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0. Do Not Dissolve in Water.</lih1> <lih1> DNA requirement : total 50-100ug of gDNA with >50 ng/ul</li> <li> Sample purity: OD260/280span id="1.8~2.0; free of protein, RNA, or other visible contamination</li> <li> The sender must provide at least one information among “Sample QC data” and “Gel image of DNA electrophoresis” </li></ul><p>2) Sender information</p><ul> <li> Name : </li> <li> Affiliation: </li> <li> Address: </li> <li> Telephone: </li> <li> Email: </li></ul><p> 3) Sample information</p><ul> <li> <p><span style="font-size: larger"><span style="font-family: ArialC2.A0Sample.28gDNA.29_Preparation"><span styleclass="linemw-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'headline"> </span></span></span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US"h2>Method of Sample type: DNA(gDNA) Preparation for Sending out</spanh2></span></span></ph1> </li> <li> <p><span style="font-size: larger"><span style="font-family: Arial"><span style="line-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'"h2> </spanh2></span></span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">Condition: <u>DNA in TE buffer or EB buffer or equivalent. Not in water</u></span> </span></span></p> </li> <li> <pb><span style="font-size: largermedium"><span styleid="font-family: Arial1..C2.A0Sample.28gDNA.29_Preparation"><span styleclass="linemw-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'headline">1. </span></span></span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">Sample QC data (</span><span style="line-height: 150%; color: red" lang="EN-US">*</span><span style="line-height: 150%; color: red; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US"> </span><span style="line-height: 150%; color: black; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">is Essential</span><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">gDNA)Preparation</span></span></span></p> </li> <li> <p><span style="font-size: larger"><span style="font-family: Arial"><span style="line-height: 150%; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: KO" lang="EN-US"><span style="mso-list: Ignore"><span style="font: 7pt 'Times New Roman'"> </span></span></spanb><span style="line-height: 150%; mso-fareast-font-family: '맑은 고딕'; mso-fareast-language: KO" lang="EN-US">Gel image of DNA electrophoresis</span></span></span> </p> </li></ul><p>4) DNA packing information</p><p> This way is only available when DNA is dissolved in TE or Tris buffer (Please read Note).<br /> If not, please pack DNA with icepack in icebox and use Fedex or DHL service.</p><ul> <li>Mark sample information on tube (concentration, sample name…what you want)</li> <li>Seal the cap of tube with parafilm well</li> <li>Wrap the tube with paper towel or equivalent</li> <li>Put into the 50 ml Falcon tube and close the cap</li> <li>Send the sample with air cushion envelop by FEDEX or DHL or equivalent.</li></ul>
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<p>Please download and refer to the following documents for information on gDNA methods of preparation and delivery1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.</p><p>1mM EDTA) or 10 mM Tris pH 8. <a href="/userfiles/DNA_sample_sheet0.doc">Sample Shipping Information </a></p><p>2.) <a href="/userfiles/Declaration_of_Shipment_for_anyone.doc">Declaration </a>Do Not Dissolve in Water</p>
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<p><b><span style="font-size: small"><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">2. Sample(gDNA) Requirement</span></span></b></p>
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<p>1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul</p>
<p> This amount is required for</p>
<p>a. Whole genome sequencing (30X)</p>
<p>b. Whole exome sequencing (50X)</p>
<p>c. Genotyping</p>
<p>2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p>
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<p><b><span style="font-size: small"><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">3. Sample(gDNA) Packing Information </span></span></b></p>
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<p>This way is only available when DNA is dissolved in TE or Tris buffer.</p>
<p>1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p>
<p>2) Mark sample information on tube (concentration, sample name…what you want)</p>
<p>3) Seal the cap of tube with parafilm well</p>
<p>4) Wrap the tube with paper towel or equivalent</p>
<p>5) Put into the 50 ml Falcon tube and close the cap</p>
<p>6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p>
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<h1><b><span style="font-size: small"><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">4. Requirement of Sample(gDNA) Information</span></span></b></h1>
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<p>1) Sample type: DNA</p>
<p>2) Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p>
<p>3) Gel image of DNA electrophoresis</p>
<p>4) Species</p>
<p>5) No. of Tubes</p>
<p>6) Concentration (ng/μl)</p>
<p>7) Volume (μl)</p>
<p>8) Total Quantity (μg)</p>
<p>9) OD260/280</p>
<p>10) OD260/230</p>
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<p><b><span style="font-size: medium">Please download and refer to the following documents for information on gDNA methods of preparation and delivery.</span></b></p>
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<p>1.<span style="color: #800000"><a href="http://papgi.org/userfiles/Method%20of%20Sample(gDNA)%20Preparation%20for%20Sending%20out.doc">Method of Sample (gDNA) Preparation for Sending out</a></span></p>
<p>2.<span style="color: #800000"><a href="http://papgi.org/userfiles/Declaration_of_Shipment_for_anyone.doc">Declaration of Shipment</a><br />
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