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13. Check each tube for the presence of the antigen. Combine tubes with high concentrations. Depending on how the antigen will be used, it may be necessary to dialyze the resulting eluted proteins to change the buffer.<br />
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14. Return the column to the starting buffer by passing 20 column volumes through the matrix. Add 0.01% Methiolate for long-term storage(4°C). <br />
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No7. Preparation of stock solution<strong><font size="3">No9. David's life chart 2<br />
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