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Cellculture

468 bytes removed, 09:00, 13 December 2006
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<p align="center">배양설비</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp;사용용도</td>
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<p align="center">탄산가스 [[배양기]](CO<sub>2</sub> incubator)</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; pH조적은 배지의 중탄산 나트륨과 CO<sub>2</sub>가스의 평형에 의해 이루어진다.<br />
&nbsp; 5% CO<sub>2</sub> - 95% air 유지<br />
&nbsp; 재킷형 CO<sub>2 </sub>incubator 주로 사용 - water 재킷(멸균수) : 온도 조절<br />
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<p align="center">배양실과 [[clean&nbsp;bench]]</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 배양실&nbsp;:&nbsp; 무균실<br />
&nbsp; clean bench : 무균조작을 위한 무균상자,&nbsp;자외선 멸균장치 포함</td>
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<p align="center">[[배양 접시]]</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; culture flask - 배양용량이 큰 배양용기, screw cap부착<br />
&nbsp; petri-dish - 조직배양용 dish(접착세포 배양), 일반세균 배양용dish(부유세포 배양)<br />
&nbsp; multi-well plate - 96 well 사용, 소량의 배양액</td>
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<p align="center">에어컨</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 온도조절</td>
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<p align="center">[[Inverted microscope]]</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 무염색 세포관찰</td>
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<p align="center">[[가압 증기 멸균기]](autoclave)</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 멸균장치</td>
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<p align="center">[[Deep freezer]]</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 동결보존 -80도 유지</td>
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<p align="center">[[원심분리기]]</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 세포와 배양액의 분리</td>
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<p align="center">냉동냉장고</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 배지, 시약 등의 보존</td>
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<p align="center">[[cover slip]]</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 염색용</td>
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<p align="center">[[auto pipette]]</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 분주</td>
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<p align="center">혈구 게시판</p>
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<td width="223" bgcolor="#ffffcc" height="27">&nbsp; 세포수 측정</td>
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