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Antibidy (항쳬)

1 byte removed, 12:07, 17 November 2006
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Ag -&gt; mouse -&gt; 1) serum 분리 -&gt; polyclonal Ab (immunochemical technique에 가장 많이 쓰임)<br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2) spleen cell 분리 -&gt; plasma cell + myeloma cell fusion -&gt; htbridoma hybridoma -&gt; select -&gt; clone들로부터 monoclonal Ab를 얻음 (tissue culture supernatant로 사용됨)<br />
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<font style="BACKGROUND-COLOR: #ffff99"> </font></font><font face="Arial"><font style="BACKGROUND-COLOR: #ffff99"><strong>Hc와 Lc의 variable region들이 Ag-binding site 형성한다</strong><br />
</font>- Ag과 결합하는 부위의 5~10 a.a (hypervariable region의) 에서 sequence heterogeity가 생김<br />
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