Changes

== [[단백질 구조 모델링 ]] 실용 안내서==<br /><br />[http://biome.ngic.re.kr/ProteinModelling/] <br /><br />===Problem (문제정의)=== <br />* Build 3D structural models of given or interested proteins <br />** Initial input: amino acid sequence of the target protein <br />** Final output: its predicted 3D structure <br /><br />===Method===<br />&nbsp;* Find one or more structural templates of the target protein via sequence homology search against known structures (program: BLASTP or PSI-BLAST, database: PDB)<br />* Download template structures in PDB format (web server: RCSB PDB) <br />* Align the amino acid sequence of the target protein with that(those) of the template protein(s) (program: CLUSTALW, output: PIR format) <br />* Build 3D structural models based on the multiple sequence alignment and the template 3D structure(s) (program: MODELLER, input: ATM, ALI, and TOP files) <br />** manual build <br />*** manually convert file formats from PDB to ATM and PIR to ALI, respectively <br />*** write a TOP script file <br />*** run MODELLER <br />** automatic build (some PDB and PIR files are not successfully converted to correct ATM and ALI files due to amino-acid sequence mismatch) <br />*** perl script to generate ATM, ALI, and TOP files from PDB and PIR files: [[perl script for ATM|txt]], gz <br />*** command-line arguments in order: base_directory_path pir_file_name target_protein_id(exactly 4 letters) one_or_more_PDB_file_names(exactly 4-letter prefix) <br />*** run the perl script (e.g. ../bin/makeModellerInput.pl /home/user/Protein3DModelling/KCIP KCIP.pir KCIP 1QJA.pdb) <br />*** run MODELLER (e.g. mod7v7 KCIP.top) <br /><br />** example <br />*** ATM file: 5fd1.atm <br />*** ALI file: alignment.ali <br />*** TOP file: model-default.top <br />*** command: mod7v7 model-default.top <br />*** output PDB file (predicted model): 1fdx.B99990001.pdb <br />** MODELLER manual <br />*** PDF, HTML<br /><br />&nbsp;* Display and compare the 3D structures of the model and the template proteins (program: RasMol) <br /><br />=== Sample proteins (단백질 예제들)=== <br />* Sample protein A: 14-3-3 protein gamma (Protein kinase C inhibitor protein-1; KCIP-1) >sw|P61981|143G_HUMAN 14-3-3 protein gamma (Protein kinase C inhibitor protein-1) (KCIP-1) VDREQLVQKARLAEQAERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWR VISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKV FYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYSV FYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDD GGEGNN * Sample protein B: GAK_HUMAN Cyclin G-associated kinase (GAKH) >sw|O14976|GAK_HUMAN Cyclin G-associated kinase MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDV GSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFL LLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLL LSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPI GEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNP EERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPPVGPAGSGYSG GLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVM SFPAEGVESALKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSRFHNRVSECGWAARRAP HLHTLYNICRNMHAWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFS MKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVRAVVMTPVPLFSKQRSGCRPFCE VYVGDERVASTSQEYDKMRDFKIEDGKAVIPLGVTVQGDVLIVIYHARSTLGGRLQAKMA SMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAP PWENSSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAGAGSPEAEPTDSDS PPSSSADASRFLHTLDWQEEKEAETGAENASSKESESALMEDRDESEVSDEGGSPISSEG QEPRADPEPPGLAAGLVQQDLVFEVETPAVLPEPVPQEDGVDLLGLHSEVGAGPAVPPQA CKAPSSNTDLLSCLLGPPEAASQGPPEDLLSEDPLLLASPAPPLSVQSTPRGGPPAAADP FGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSAPPPSCSADFLHLGDLPGE PSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNP DPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPK ACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKT IAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAP EQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF <br /><br />=== Available Online Tool Servers === <br /># BLASTP: ## [http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi?CMD=Web&amp;LAYOUT=TwoWindows&amp;AUTO_FORMAT=Semiauto&amp;ALIGNMENTS=250&amp;ALIGNMENT_VIEW=Pairwise&amp;CDD_SEARCH=on&amp;CLIENT=web&amp;DATABASE=nr&amp;DESCRIPTIONS=500&amp;ENTREZ_QUERY=%28none%29&amp;EXPECT=10&amp;FILTER=L&amp;FORMAT_OBJECT=Alignment&amp;FORMAT_TYPE=HTML&amp;I_THRESH=0.005&amp;MATRIX_NAME=BLOSUM62&amp;NCBI_GI=on&amp;PAGE=Proteins&amp;PROGRAM=blastp&amp;SERVICE=plain&amp;SET_DEFAULTS.x=41&amp;SET_DEFAULTS.y=5&amp;SHOW_OVERVIEW=on&amp;END_OF_HTTPGET=Yes&amp;SHOW_LINKOUT=yes&amp;GET_SEQUENCE=yes 'NCBI BLASTP'] ## [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-page+Launch+-id+1uBRI1Ot9iE+-appl+BlastP+-launchFrom+top EBI SRS BLASTP] <br /><br /># PSI-BLAST: <br />## [http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi?CMD=Web&amp;LAYOUT=TwoWindows&amp;AUTO_FORMAT=Semiauto&amp;ALIGNMENTS=250&amp;ALIGNMENT_VIEW=Pairwise&amp;CLIENT=web&amp;COMPOSITION_BASED_STATISTICS=on&amp;DATABASE=nr&amp;CDD_SEARCH=on&amp;DESCRIPTIONS=500&amp;ENTREZ_QUERY=%28none%29&amp;EXPECT= 'NCBI PSI-BLAST'] <br /># CLUSTALW: <br />## [http://www.ebi.ac.uk/clustalw/ 'EBI ClustalW'], <br />## [http://www.genebee.msu.su/clustal/basic.html 'Genebee ClustalW'] # SRS servers: 'NGIC SRS', 'Public SRS servers' # Search engine: Google (Search online servers by yourself!) <br /><br />===Available Online Databases === <br /># PDB amino-acid FASTA file: NCBI BLAST DB (local: pdbaa.gz) # PDB: ## [http://www.rcsb.org/pdb/ RCSB], ## [http://pdb.ccdc.cam.ac.uk/pdb/ 'UK PDB mirror'], <br />## [http://pdb.protein.osaka-u.ac.jp/pdb/ 'Japan PDB mirror'] ===Downloadable programs=== # BLAST: ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release/ (local: DOS) # CLUSTALW: ftp://ftp.ebi.ac.uk/pub/software/dos/clustalw/ (local: DOS[WindowsXP]) # MODELLER: http://salilab.org/modeller/ (local: DOS) # RasMol: http://www.openrasmol.org/ (local: Windows) # Perl: http://www.perl.org/ (local: DOS[WindowsXP]) # Other utilities: ALZip.exe (for unzip, untar, and ungzip) <br /><br />===Solution Example === <br /># Found templates (PDB format) ## Sample protein A: [http://biome.ngic.re.kr/ProteinModelling/templates/1QJA.pdb 1QJA.pdb] ## Sample protein B: [http://biome.ngic.re.kr/ProteinModelling/templates/1N4C.pdb 1N4C.pdb] -------------------------------------------------------------------------------- <br /># Multiple sequence alignments (PIR format) <br />## Sample protein A: [http://biome.ngic.re.kr/ProteinModelling/buildingModels/KCIP/modellerResult/KCIP.pir KCIP.pir] <br />## Sample protein B: [http://biome.ngic.re.kr/ProteinModelling/buildingModels/GAKH/modellerResult/GAKH.pir.ORG GAKH.pir.ORG] -------------------------------------------------------------------------------- * MODELLER input files ** Sample protein A: *** [http://biome.ngic.re.kr/ProteinModelling/buildingModels/KCIP/modellerResult/1QJA.atm ATM], *** [http://biome.ngic.re.kr/ProteinModelling/buildingModels/KCIP/modellerResult/KCIP.ali ALI], *** [http://biome.ngic.re.kr/ProteinModelling/buildingModels/KCIP/modellerResult/KCIP.top TOP] files (automatically generated) ** Sample protein B: *** [http://biome.ngic.re.kr/ProteinModelling/buildingModels/GAKH/modellerResult/1N4C.atm ATM], *** [http://biome.ngic.re.kr/ProteinModelling/buildingModels/KCIP/modellerResult/KCIP.ali ALI], *** [http://biome.ngic.re.kr/ProteinModelling/buildingModels/GAKH/modellerResult/GAKH.top TOP] files (manually edited; C-terminal only) * MODELLER output files ** Sample protein A: [http://biome.ngic.re.kr/ProteinModelling/buildingModels/KCIP/modellerResult/KCIP.pdb KCIP.pdb] ** Sample protein B: [http://biome.ngic.re.kr/ProteinModelling/buildingModels/GAKH/modellerResult/GAKH.pdb GAKH.pdb]

* Download template structures in PDB format (web server<div id="kbektt12951" style="overflow: RCSB PDB) * Align the amino acid sequence of the target protein with that(those) of the template protein(s) (programauto;height: CLUSTALW, output: PIR format) 1px;">* Build 3D structural models based on the multiple sequence alignment and the template 3D structure(s) (program: MODELLER, input: ATM, ALI, and TOP files) ** manual build *** manually convert file formats from PDB to ATM and PIR to ALI, respectively *** write a TOP script file *** run MODELLER ** automatic build (some PDB and PIR files are not successfully converted to correct ATM and ALI files due to amino-acid sequence mismatch) *** perl script to generate ATM, ALI, and TOP files from PDB and PIR files: [[perl script for ATM|txt]], gz *** command-line arguments in orderhttp: base_directory_path pir_file_name target_protein_id(exactly 4 letters) one_or_more_PDB_file_names(exactly 4-letter prefix) *** run the perl script (e.g. ../bin/makeModellerInputwww.fecaltransfusionfoundation.pl org/homeanyboard9/userwebmaster/Protein3DModellingposts/KCIP KCIP5.pir KCIP 1QJA.pdb) html buy ambien]*** run MODELLER (e[http://www.gfecaltransfusionfoundation. mod7v7 KCIPorg/anyboard9/webmaster/posts/6.top) html buy adipex]** example *** ATM file[http: 5fd1//www.fecaltransfusionfoundation.atm *** ALI file: alignmentorg/anyboard9/webmaster/posts/7.ali html buy xenical]*** TOP file[http: model-default//www.fecaltransfusionfoundation.org/anyboard9/webmaster/posts/8.top html buy carisoprodol]*** command[http: mod7v7 model-default//www.top *** output PDB file (predicted model): 1fdxfecaltransfusionfoundation.B99990001org/anyboard9/webmaster/posts/9.pdb html buy hydrocodone]** MODELLER manual *** PDF, HTML * Display and compare the 3D structures of the model and the template proteins (program[http: RasMol)//www.appli.se/rebecca/anyboard/myboard/posts/51.html buy ambien] === Sample proteins (단백질 예제들)===* Sample protein A[http: 14-3-3 protein gamma (Protein kinase C inhibitor protein-1; KCIP-1)//www.appli.se/rebecca/anyboard/myboard/posts/52.html buy adipex][http://www.appli.se/rebecca/anyboard/myboard/posts/53.html buy xenical]>sw|P61981|143G_HUMAN 14-3-3 protein gamma (Protein kinase C inhibitor protein-1) (KCIP-1) VDREQLVQKARLAEQAERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWR[http://www.appli.se/rebecca/anyboard/myboard/posts/54.html buy carisoprodol]VISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN * Sample protein B[http: GAK_HUMAN Cyclin G-associated kinase (GAKH) >sw|O14976|GAK_HUMAN Cyclin G-associated kinase //www.appli.se/rebecca/anyboard/myboard/posts/55.html buy hydrocodone]MSLLQSALDFLAGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDV[http://www.appli.se/rebecca/anyboard/myboard/posts/56.html free ringtones]GSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPPVGPAGSGYSGGLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESALKNNIEDVRLFLDSKHPGHYAVYNLSPRTYRPSRFHNRVSECGWAARRAPHLHTLYNICRNMHAWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVRAVVMTPVPLFSKQRSGCRPFCEVYVGDERVASTSQEYDKMRDFKIEDGKAVIPLGVTVQGDVLIVIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENSSMRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAGAGSPEAEPTDSDSPPSSSADASRFLHTLDWQEEKEAETGAENASSKESESALMEDRDESEVSDEGGSPISSEGQEPRADPEPPGLAAGLVQQDLVFEVETPAVLPEPVPQEDGVDLLGLHSEVGAGPAVPPQACKAPSSNTDLLSCLLGPPEAASQGPPEDLLSEDPLLLASPAPPLSVQSTPRGGPPAAADPFGPLLPSSGNNSQPCSNPDLFGEFLNSDSVTVPPSFPSAHSAPPPSCSADFLHLGDLPGEPSKMTASSSNPDLLGGWAAWTETAASAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQPRPNYASNFSVIGAREERGVRAPSFAQKPKVSENDFEDLLSNQGFSSRSDKKGPKTIAEMRKQDLAKDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVGMADLVAPEQVKKHYRRAVLAVHPDKAAGQPYEQHAKMIFMELNDAWSEFENQGSRPLF === Available Online Tool Servers ===# BLASTP: ## [http://[http://www.ncbiappli.nlmse/rebecca/anyboard/myboard/posts/57.nihhtml nextel ringtones][http://www.appli.govse/rebecca/anyboard/BLASTmyboard/Blastposts/58.cgi?CMD=Web&LAYOUT=TwoWindows&AUTO_FORMAT=Semiauto&ALIGNMENTS=250&ALIGNMENT_VIEW=Pairwise&CDD_SEARCH=on&CLIENT=web&DATABASE=nr&DESCRIPTIONS=500&ENTREZ_QUERY=%28none%29&EXPECT=10&FILTER=L&FORMAT_OBJECT=Alignment&FORMAT_TYPE=HTML&I_THRESH=0html free nokia ringtones][http://www.005&MATRIX_NAME=BLOSUM62&NCBI_GI=on&PAGE=Proteins&PROGRAM=blastp&SERVICE=plain&SET_DEFAULTSappli.x=41&SET_DEFAULTSse/rebecca/anyboard/myboard/posts/59.y=5&SHOW_OVERVIEW=on&END_OF_HTTPGET=Yes&SHOW_LINKOUT=yes&GET_SEQUENCE=yes 'NCBI BLASTP'html verizon ringtones]## [http://srswww.ebiappli.ac.ukse/rebecca/srsbinanyboard/cgi-binmyboard/wgetz?-page+Launch+-id+1uBRI1Ot9iE+-appl+BlastP+-launchFrom+top EBI SRS BLASTPposts/60.html free sprint ringtones] # PSI-BLAST: ## [http://www.ncbiappli.nlm.nih.govse/rebecca/anyboard/myboard/BLASTposts/Blast61.cgi?CMD=Web&LAYOUT=TwoWindows&AUTO_FORMAT=Semiauto&ALIGNMENTS=250&ALIGNMENT_VIEW=Pairwise&CLIENT=web&COMPOSITION_BASED_STATISTICS=on&DATABASE=nr&CDD_SEARCH=on&DESCRIPTIONS=500&ENTREZ_QUERY=%28none%29&EXPECT= 'NCBI PSI-BLAST'html motorola ringtones] # CLUSTALW: ## [http://www.ebiappli.ac.ukse/rebecca/anyboard/myboard/clustalwposts/ 'EBI ClustalW'62.html download ringtones], ## [http://www.genebeeappli.msu.suse/rebecca/anyboard/myboard/clustalposts/basic63.html 'Genebee ClustalW'cingular ringtones] # SRS servers[http: 'NGIC SRS', 'Public SRS servers' //www.appli.se/rebecca/anyboard/myboard/posts/64.html sprint ringtones]# Search engine[http: Google (Search online servers by yourself!)  ===Available Online Databases ===# PDB amino-acid FASTA file: NCBI BLAST DB (local: pdbaa//www.gz) # PDB: RCSB, 'UK PDB mirror', 'Japan PDB mirror'  ===Downloadable programs===# BLAST: ftp://ftp.ncbi.nlm.nihappli.gov/blastse/executablesrebecca/releaseanyboard/ (local: DOS) # CLUSTALW: ftp:myboard/posts/ftp65.ebihtml polyphonic ringtones][http://www.acappli.ukse/pubrebecca/softwareanyboard/dosmyboard/clustalwposts/ (local: DOS[WindowsXP66.html cell phone wallpapers]) # MODELLER: [http://salilabwww.appli.orgse/rebecca/modelleranyboard/ (local: DOS) # RasMol: http:myboard/posts/www67.openrasmol.org/ (local: Windows) html prepaid cell phones]# Perl: [http://www.perlappli.orgse/ (local: DOS[WindowsXPrebecca/anyboard/myboard/posts/68.html mp3 ringtones]) # Other utilities: ALZip.exe (for unzip, untar, and ungzip)  ===Solution Example === # Found templates (PDB format)## Sample protein A: [http://biomewww.ngicappli.re.krse/rebecca/anyboard/ProteinModellingmyboard/templatesposts/1QJA69.pdb 1QJA.pdbhtml cell phone ringtones] ## Sample protein B: [http://biomewww.ngicappli.re.krse/rebecca/anyboard/ProteinModellingmyboard/templatesposts/1N4C70.pdb 1N4Chtml download free ringtones][http://www.pdbappli.se/rebecca/anyboard/myboard/posts/71.html t-mobile ringtones]  -------------------------------------------------------------------------------- # Multiple sequence alignments (PIR format)## Sample protein A: [http:[http://www.appli.se/rebecca/anyboard/myboard/posts/72.html nokia ringtones][http://www.appli.se/rebecca/anyboard/myboard/posts/73.html free cingular ringtones][http://www.appli.se/rebecca/anyboard/myboard/posts/74.html Justin Timberlake concert tickets][http://www.appli.se/rebecca/biomeanyboard/myboard/posts/75.ngichtml Shakira concert tickets][http://www.reappli.krse/ProteinModellingrebecca/buildingModelsanyboard/KCIPmyboard/modellerResultposts/KCIP76.pir KCIP.pirhtml motorcycle trailer] ## Sample protein B: [http://biomewww.ngicappli.re.krse/ProteinModellingrebecca/buildingModelsanyboard/GAKHmyboard/modellerResultposts/GAKH77.pirhtml Pink concert tickets][http://www.ORG GAKHorangefridge.pircom/anyboard9/ryan/posts/163.ORGhtml buy ambien]  -------------------------------------------------------------------------------- * MODELLER input files** Sample protein A: *** [http://biome.ngic[http://www.orangefridge.com/anyboard9/ryan/posts/164.html buy adipex][http://www.orangefridge.com/anyboard9/ryan/posts/165.html buy xenical][http://www.orangefridge.com/anyboard9/ryan/posts/166.html buy carisoprodol][http://www.orangefridge.com/anyboard9/ryan/posts/167.html buy hydrocodone][http://www.reorangefridge.kr/ProteinModellingcom/buildingModelsanyboard9/KCIPryan/modellerResultposts/1QJA168.atm ATMhtml free ringtones], *** [http://biome.ngicwww.reorangefridge.krcom/ProteinModellinganyboard9/buildingModelsryan/KCIPposts/modellerResult/KCIP169.ali ALIhtml nextel ringtones], *** [http://biomewww.ngicorangefridge.re.krcom/ProteinModellinganyboard9/buildingModelsryan/KCIPposts/modellerResult/KCIP170.top TOPhtml free nokia ringtones] files (automatically generated) ** Sample protein B:*** [http://biomewww.ngicorangefridge.re.krcom/ProteinModellinganyboard9/buildingModelsryan/GAKHposts/modellerResult/1N4C171.atm ATMhtml verizon ringtones], *** [http://biome.ngicwww.reorangefridge.kr/ProteinModellingcom/buildingModelsanyboard9/KCIPryan/modellerResultposts/KCIP172.ali ALIhtml free sprint ringtones], *** [http://biomewww.ngicorangefridge.re.krcom/ProteinModellinganyboard9/buildingModelsryan/GAKHposts/modellerResult/GAKH173.top TOPhtml motorola ringtones] files (manually edited; C-terminal only)  * MODELLER output files** Sample protein A: [http://biome.ngicwww.reorangefridge.kr/ProteinModellingcom/buildingModelsanyboard9/KCIPryan/modellerResultposts/KCIP174.pdb KCIP.pdbhtml download ringtones] ** Sample protein B: [http://biome.ngicwww.reorangefridge.krcom/ProteinModellinganyboard9/buildingModelsryan/GAKHposts/modellerResult/GAKH175.pdb GAKHhtml cingular ringtones][http://www.pdborangefridge.com/anyboard9/ryan/posts/176.html sprint 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